Kato Shogo
Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan.
Methods Mol Biol. 2019;1924:19-26. doi: 10.1007/978-1-4939-9015-3_2.
By the nineteenth century, root climbers and adhesive-tendril climbers were known to exhibit negative phototropism. Negative phototropism is shared by various plant species belonging to many taxonomic families and is considered to be an outcome of parallel evolution. Through negative phototropism, lianas search for supporting hosts; however, compared with positive phototropism, which occurs during germination, there is little research on the properties of negative phototropism. This chapter presents a technique for quantifying negative phototropism in root climbers and adhesive-tendril climbers, which involves casting light on one side of a liana shoot and measuring the coordinates of the shoot tip and the angle of curvature of the entire shoot relative to the gradient of the light conditions.
到19世纪时,人们已知根攀缘植物和具粘性卷须的攀缘植物表现出负向光性。负向光性为许多分类科的各种植物物种所共有,被认为是平行进化的结果。通过负向光性,藤本植物寻找支撑宿主;然而,与种子萌发时发生的正向光性相比,关于负向光性特性的研究很少。本章介绍了一种量化根攀缘植物和具粘性卷须的攀缘植物负向光性的技术,该技术包括在藤本植物茎的一侧照射光线,并测量茎尖的坐标以及整个茎相对于光照条件梯度的曲率角度。