Ueda Haruko, Hara-Nishimura Ikuko
Department of Biology, Faculty of Science and Engineering, Konan University, Kobe, Japan.
Methods Mol Biol. 2019;1924:215-221. doi: 10.1007/978-1-4939-9015-3_18.
Although plant organ segments bend in response to environmental stimuli such as gravity and light, they stop bending and subsequently straighten during the course of tropic responses. The straightening phenomenon can clearly be observed by setting the bent organs under microgravity and dark conditions. It has recently become clear that the straightening mechanism requires the activity of the actin-myosin XI cytoskeleton. A clinostat device makes it possible to simulate microgravity conditions by counteracting the Earth's unilateral gravitational pull. Here, we describe a method for assessing the straightening ability of organs by clinostat analysis using Arabidopsis thaliana inflorescence stems of actin and myosin xi mutants as examples.
尽管植物器官段会因重力和光照等环境刺激而弯曲，但在向性反应过程中它们会停止弯曲并随后伸直。通过将弯曲的器官置于微重力和黑暗条件下，可以清楚地观察到伸直现象。最近已经明确，伸直机制需要肌动蛋白 - 肌球蛋白XI细胞骨架的活性。回转器装置可以通过抵消地球的单向引力来模拟微重力条件。在这里，我们以拟南芥肌动蛋白和肌球蛋白xi突变体的花序茎为例，描述一种通过回转器分析评估器官伸直能力的方法。
