Yamamoto Kotaro T, Haga Ken
Department of Biological Sciences, Faculty of Science, Hokkaido University, Sapporo, Japan.
Department of Applied Chemistry, Faculty of Fundamental Engineering, Nippon Institute of Technology, Miyashiro, Saitama, Japan.
Methods Mol Biol. 2019;1924:223-234. doi: 10.1007/978-1-4939-9015-3_19.
Tropic responses in plants have usually been studied by measuring changes in the deflection angle of the organ tip. However, the measurement of other geometric parameters, such as curvature along the entire length, may give us better understanding of tropic responses, particularly in shoots. Here, we describe methods for obtaining quantitative measurements of local curvature and other parameters based on digital images of bending Arabidopsis hypocotyls using the free software packages, ImageJ and R.
植物的向性反应通常是通过测量器官尖端偏转角的变化来研究的。然而，测量其他几何参数，如沿整个长度的曲率，可能会让我们更好地理解向性反应，尤其是在芽中。在这里，我们描述了使用免费软件包ImageJ和R，基于拟南芥弯曲下胚轴的数字图像获得局部曲率和其他参数定量测量的方法。
