Inoue Shinichi, Matsuki Yoshinori, Mori Masaya
Rinsho Byori. 2016 Nov;64(11):1215-1219.
It is well known that the antigenicity of unstained sections put on a slide glass decreases when the slides are stored at room temperature. Several methods have been reported to prevent reduced antigenicity, such as storing in a dark place at 4°C, covering sections with paraffin or a sheet, and storing in a deep freezer at -80°C. The aim of the present study is to determine how long unstained sections can be stored in a refriger- ator (ie, 1 week, 2 weeks, 1 month and 2 months). Each section was well stained and equal to the day 0 section for CD3, CD20, bcl-2, bcl-6, ER, PgR, HER2, Ki-67, chromogranin-A, synaptophysin, CK5, CK7, CK20, and TTF-1. In conclusion, unstained sections can be used for immunostaining after at least 2 months of storage, and preparing several control sections and storing them in a refrigerator is useful for minimizing control block loss. [Original].
众所周知，当载玻片在室温下储存时，放在载玻片上的未染色切片的抗原性会降低。已经报道了几种防止抗原性降低的方法，例如在4°C的暗处储存、用石蜡或薄片覆盖切片以及在-80°C的深冻箱中储存。本研究的目的是确定未染色切片可以在冰箱中储存多长时间（即1周、2周、1个月和2个月）。对于CD3、CD20、bcl-2、bcl-6、ER、PgR、HER2、Ki-67、嗜铬粒蛋白A、突触素、CK5、CK7、CK20和TTF-1，每个切片染色良好且与第0天的切片相当。总之，未染色切片在储存至少2个月后可用于免疫染色，制备几个对照切片并将它们储存在冰箱中有助于最大限度地减少对照块的损失。[原文]
