Tyurin M V, Kryukov V Yu, Yaroslavtseva O N, Elisaphenko E A, Dubovskiy I M, Glupov V V
Zh Evol Biokhim Fiziol. 2016 Mar;52(2):226-232.
A comparative investigation of humoral and cellular immune response in larvae of Colorado potato beetle Leptinotarsa decemlineata was conducted under development of mycoses caused by entomopatho- genic fungi Metarhizium robertsii, M. brunneum and M. pemphigi. The larvae were found highly suscep- tible to M. robertsii, M. brunneum and less susceptible to M. pemphigi. The susceptibility to the fungi was not correlated with the rate of conidia germination in epicuticular extracts of larvae. A non-specific for Colorado beetle pathogen M. pemphigi did not cause significant changes in the immune response and did not result in colonization of larvae hemocoel. Infection with M. robertsi and M. brunneum led to an increase in total hemocyte count at the initial stages of mycoses (day 2) followed by a sharp decrease on day 3. The strongest decrease was observed for the immunocompetent cells - plasmatocytes and granu- locytes. Enhanced phenoloxidase activity in hemolymph and cuticle was found on days 2 and 3 after in- fection. These changes in immune reactions correlated with the level of virulence of the strains. Thus, the immune response in Colorado potato beetle larvae is an important factor determining differences in the development of mycoses caused by different Metarhizium species.
在由昆虫病原真菌罗伯茨绿僵菌、布氏绿僵菌和膨腹绿僵菌引起的真菌病发展过程中，对科罗拉多马铃薯甲虫幼虫的体液免疫和细胞免疫反应进行了比较研究。发现幼虫对罗伯茨绿僵菌、布氏绿僵菌高度敏感，对膨腹绿僵菌较不敏感。对真菌的敏感性与幼虫表皮提取物中分生孢子的萌发率无关。对科罗拉多马铃薯甲虫无特异性的病原菌膨腹绿僵菌不会引起免疫反应的显著变化，也不会导致幼虫血腔定殖。感染罗伯茨绿僵菌和布氏绿僵菌在真菌病初期（第2天）导致总血细胞计数增加，随后在第3天急剧下降。免疫活性细胞——浆细胞和粒细胞的下降最为明显。感染后第2天和第3天，血淋巴和表皮中的酚氧化酶活性增强。这些免疫反应的变化与菌株的毒力水平相关。因此，科罗拉多马铃薯甲虫幼虫的免疫反应是决定不同绿僵菌物种引起的真菌病发展差异的重要因素。
