[Proteasome and calpain activities in colon cancer: the relation with metastasis and prognosis].

The chymotrypsin-like total proteasome activity and activity of 26S and 20S pools of proteasomes as well as calpain activitiy were found to be significantly increased in colon cancer tissue as compared to normal colon tissue. Patients with stage T2-4N1-3M0-lcolon cancer had higher activity level of the 26/20S proteasome and total calpain activities than patients with stage T2-4NOMO-1 colon cancer. The chymotrypsin-like total activity of proteasomes and the total activity of caplains in patients with stage T2-4N0-3M1 colon cancer were re- spectively 1.6 and 2.0-fold higher compared to those in patients with stage T2-4N0-3M0 colon cancer. Survival analysis of patients with colon cancer showed that the high level of chymotrypsin-like protesome activity in colon cancer and normal colon tissues (more than 72-1000 units/mg protein and 35-1000 units/mg protein, respectively) and the high level of total calpain activity in colon cancer and normal colon tissues (more than 100.5-1000 Units/mg protein and 52-1000 Units/ mg protein, respectively) were unfavorable factors in terms of 2-year metastasis-free survival.