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Quantitative determination of cross-linkage of bacteriophage DNA and protein by ionizing radiation.

作者信息

Hawkins R B

出版信息

Int J Radiat Biol Relat Stud Phys Chem Med. 1978 May;33(5):425-41. doi: 10.1080/09553007814550351.

Abstract

Coliphage T7 was dissolved in tryptone broth and exposted to 60C gamma-radiation. Cross-linkage of DNA and protein of the virion was assayed using phenol-water countercurrent distribution. The results are interpreted in terms of a statistical model of cross-linkage and double-strand breaks. It was found that protein--DNA cross-links accumulate linearly with dose at a rate of o.74 X 10(-11) cross-links per rad per nucleotide pair, which is of the order of 5 per cent of the formation rate of double-strand breaks.

摘要

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