Mäder M, Dickmann U
Neurologische Klinik der Universität Göttingen.
J Clin Chem Clin Biochem. 1988 Dec;26(12):857-61. doi: 10.1515/cclm.1988.26.12.857.
Choline acetyltransferase (EC 2.3.1.6) was studied in human cerebrospinal fluid and blood. Sensitivity, precision and reproducibility of the radioactive assay were evaluated with a homogenate from pig brain and with partially purified enzymes from bovine brain and human placenta. Contrary to other reports, with the assay used in the present study, choline acetyltransferase activity was not detectable in the cerebrospinal fluid or blood of 50 patients with various neurological disorders. Only a very slight but significant non-enzymatic formation of a radioactive product by concentrated cerebrospinal fluid was observed. Nonenzymatic but enzyme-like formation of acetylcholine by thiol reagents and other compounds is discussed.
对人脑脊液和血液中的胆碱乙酰转移酶（EC 2.3.1.6）进行了研究。使用猪脑匀浆以及来自牛脑和人胎盘的部分纯化酶对放射性测定法的灵敏度、精密度和可重复性进行了评估。与其他报告相反，在本研究中所使用的测定法下，50名患有各种神经系统疾病的患者的脑脊液或血液中未检测到胆碱乙酰转移酶活性。仅观察到浓缩脑脊液有非常轻微但显著的放射性产物非酶形成。讨论了硫醇试剂和其他化合物非酶但类似酶的乙酰胆碱形成情况。
