Kil Eui-Joon, Byun Hee-Seong, Kim Sunhoo, Cho Seungchan, Cho Sungrae, Roh Kangsan, Lee Kyeong-Yeoll, Choi Hong-Soo, Kim Chang-Seok, Lee Sukchan
Department of Genetic Engineering and The Institute of Life Science and Technology, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
Department of Genetic Engineering, Sungkyunkwan University.
Plant Dis. 2015 May;99(5):588-592. doi: 10.1094/PDIS-04-14-0352-RE. Epub 2015 Apr 30.
Tomato yellow leaf curl virus (TYLCV), one of the most serious plant viruses in tropical and subtropical regions, is transmitted to host plants by the vector insect Bemisia tabaci. In order to control TYLCV, it is important to identify weed hosts for overwintering TYLCV. Stellaria aquatica, a winter-hardy weed, was found growing with TYLCV-infected tomato plants in greenhouse production. TYLCV was detected in S. aquatica plants by polymerase chain reaction and Southern blot hybridization analysis. The intergenic region nucleotide sequences amplified from TYLCV-infected tomato plants, TYLCV-viruliferous whiteflies, and S. aquatica were identical. During winter (December to February), TYLCV-viruliferous whiteflies and TYLCV-infected tomato plants were removed or absent from greenhouses. However, S. aquatica plants were observed over a period of 10 months from August to May in such greenhouses, and TYLCV was consistently detected in some of these plants. To investigate the transmission of TYLCV from TYLCV-infected S. aquatica plants to healthy tomato plants by whiteflies, TYLCV-infected S. aquatica plants were transplanted to pots in cages with nonviruliferous whiteflies and healthy tomato plants. After 4 weeks, tomato plants developed typical TYLCV disease symptoms, and TYLCV was detected in both whiteflies and tomato plants. These results show that S. aquatica can act as a winter-hardy reservoir for TYLCV, and suggest that this weed could play an important role in overwintering of TYLCV in tomato greenhouses.
番茄黄化曲叶病毒（TYLCV）是热带和亚热带地区最严重的植物病毒之一，通过媒介昆虫烟粉虱传播到寄主植物上。为了控制TYLCV，识别其越冬的杂草寄主很重要。硬叶女娄菜是一种耐寒杂草，在温室生产中发现它与感染TYLCV的番茄植株一起生长。通过聚合酶链反应和Southern杂交分析在硬叶女娄菜植株中检测到了TYLCV。从感染TYLCV的番茄植株、带毒烟粉虱和硬叶女娄菜中扩增出的基因间隔区核苷酸序列是相同的。在冬季（12月至2月），温室中移除了带毒烟粉虱和感染TYLCV的番茄植株，或者这些植株不存在。然而，在这样的温室中从8月到5月的10个月期间观察到了硬叶女娄菜植株，并且在其中一些植株中持续检测到TYLCV。为了研究烟粉虱将TYLCV从感染TYLCV的硬叶女娄菜植株传播到健康番茄植株的情况，将感染TYLCV的硬叶女娄菜植株移植到装有无毒烟粉虱和健康番茄植株的笼子中的花盆里。4周后，番茄植株出现了典型的TYLCV病害症状，并且在烟粉虱和番茄植株中都检测到了TYLCV。这些结果表明硬叶女娄菜可以作为TYLCV的耐寒储存寄主，并表明这种杂草可能在番茄温室中TYLCV的越冬过程中起重要作用。
