Obstetrics and Gynecology, Roy J and Lucille A Carver College of Medicine, University of Iowa, Iowa City, IA, USA.
Obstetrics and Gynecology, University of Iowa Hospitals and Clinics, Iowa City, IA, USA.
J Matern Fetal Neonatal Med. 2020 Sep;33(18):3116-3124. doi: 10.1080/14767058.2019.1569614. Epub 2019 Jan 30.
To identify microRNAs (miRNAs) differentially expressed in plasma exosomes collected in women diagnosed with preeclampsia compared with women with uncomplicated pregnancies. Exosomes were purified from plasma samples obtained at each trimester from four women subsequently diagnosed with preeclampsia and from five matched healthy controls. RNA was purified from the exosomes, and expression of 368 miRNAs was profiled using A-Set TaqMan low density array (TLDA). One-third of the 368 miRNAs profiled are not expressed in exosomes. Further, those that are not expressed tend to be evolutionarily younger and have a significantly different mature sequence signature than do miRNAs that are expressed in exosomes. Among miRNAs that are expressed in exosomes, a total of eight (, , and ) were found to display statistically significant differential expression between women who developed preeclampsia as compared with those who did not. Moreover, half of these miRNAs (, , and ) displayed statistically significant differential expression in the first trimester. Not all miRNAs are expressed in exosomes. Those that tend to be evolutionarily older and have a significantly different mature sequence signature than those that are not. A few exosome-expressed miRNAs do display expression patterns in women subsequently diagnosed with preeclampsia that are significantly different than in women having an uncomplicated and, among these, several appear in the first trimester. These miRNAs are potential early markers of preeclampsia risk.
为了鉴定在患有先兆子痫的女性的血浆外泌体中与正常妊娠女性相比差异表达的 microRNAs（miRNAs）。从随后被诊断为先兆子痫的 4 名女性和 5 名匹配的健康对照者的每个孕期的血浆样本中纯化外泌体。从外泌体中纯化 RNA，并使用 A-Set TaqMan 低密度阵列（TLDA）对 368 种 miRNAs 的表达进行分析。在分析的 368 种 miRNAs 中，有三分之一在外泌体中不表达。此外，那些不表达的 miRNA 往往比在外泌体中表达的 miRNA 更具进化年轻性，并且具有明显不同的成熟序列特征。在外泌体中表达的 miRNAs 中，总共发现 8 种（、和）在患有先兆子痫的女性与未患有先兆子痫的女性之间显示出统计学上显著的差异表达。此外，其中一半（、和）在孕早期显示出统计学上显著的差异表达。并非所有的 miRNAs 都在外泌体中表达。那些往往比不表达的 miRNA 更具进化年轻性，并且具有明显不同的成熟序列特征。少数外泌体表达的 miRNAs 在随后被诊断为患有先兆子痫的女性中表现出与在没有并发症的女性中明显不同的表达模式，其中一些出现在孕早期。这些 miRNAs 是先兆子痫风险的潜在早期标志物。
