一种用于共聚焦显微镜成像拟南芥花组织的简单方案。

A Simple Protocol for Imaging Floral Tissues of Arabidopsis with Confocal Microscopy.

作者信息

Gómez-Felipe Andrea, de Folter Stefan

机构信息

Unidad de Genómica Avanzada, Laboratorio Nacional de Genómica para la Biodiversidad (LANGEBIO), Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Irapuato, Guanajuato, Mexico.

出版信息

Methods Mol Biol. 2019;1932:187-195. doi: 10.1007/978-1-4939-9042-9_14.

DOI:10.1007/978-1-4939-9042-9_14

PMID:30701501

Abstract

We present a simple protocol to image floral tissues with confocal laser scanning microscopy (CLSM). Recently, new imaging techniques have emerged that improve the image quality of plant tissues. In this protocol, as an example, we focus on the fluorescence detection of the miRNA MIR164c precursor. Briefly, the method involves tissue clearing, cell wall staining, and the visualization of fluorescence in tissues in young floral buds of Arabidopsis with CLSM with the use of water dipping lenses.

摘要

我们展示了一种使用共聚焦激光扫描显微镜（CLSM）对花组织进行成像的简单方案。最近，出现了一些新的成像技术，可提高植物组织的图像质量。在本方案中，作为示例，我们聚焦于对miRNA MIR164c前体的荧光检测。简而言之，该方法包括组织透明化、细胞壁染色，以及使用水浸物镜通过CLSM对拟南芥幼花芽中的组织荧光进行可视化。