Li Shengben
Nanjing Agricultural University, Nanjing, China.
Methods Mol Biol. 2019;1932:317-333. doi: 10.1007/978-1-4939-9042-9_23.
Polysomes play central roles in protein synthesis. Free polysomes are responsible for the translation of cytosolic and nuclear proteins, and the membrane-bound polysomes are responsible for the translation of transmembrane and secreted proteins. Accumulative evidence from recent studies shows that microRNAs are highly enriched on polysomes to regulate the expression of their own and target mRNAs. Here, we describe experimental protocols for the isolation of total and membrane-bound polysomes and the detection of the associated microRNAs and AGO1 proteins.
多核糖体在蛋白质合成中起核心作用。游离多核糖体负责细胞质和核蛋白的翻译，而膜结合多核糖体负责跨膜蛋白和分泌蛋白的翻译。最近研究的累积证据表明，微小RNA在多核糖体上高度富集，以调节其自身和靶mRNA的表达。在这里，我们描述了分离总多核糖体和膜结合多核糖体以及检测相关微小RNA和AGO1蛋白的实验方案。
