Roufidou Chrysoula, Borg Bertil
Department of Zoology, Stockholm University, Stockholm, Sweden.
J Fish Biol. 2019 Mar;94(3):446-457. doi: 10.1111/jfb.13915.
The ovarian fluid properties of the three-spined stickleback Gasterosteus aculeatus were studied in overripe and non-overripe ovulated female sticklebacks and in females that were implanted with Silastic capsules containing testosterone (T), oestradiol (E2), 17,20β-dihydroxypregn-4-en-3-one (17,20β-P) or progesterone (P4) into the abdominal cavity. Overripe females had less ovarian fluid than non-overripe females, but with higher dry mass, higher protein concentration and lower viscosity. T and 17,20β-P increased the amount of ovarian fluid and the fluid protein concentration was increased by 17,20β-P. 1-D sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) showed that ovarian fluid contains several proteins, with high individual variability but with no consistent differences between groups. Some of the ovarian fluid proteins appeared to correspond to proteins from the eggs. The results suggest that secretion of ovarian fluid may be influenced by steroid hormones and that changes in its properties are related to the overripening of ovulated eggs. In at least some respects it appears that the changes in the ovarian fluid is a result, rather than the cause of overripening.
在过熟和未过熟排卵的雌性三刺鱼以及腹腔内植入含睾酮（T）、雌二醇（E2）、17,20β - 二羟基孕 - 4 - 烯 - 3 - 酮（17,20β - P）或孕酮（P4）的硅橡胶胶囊的雌性三刺鱼中，研究了三刺鱼（Gasterosteus aculeatus）的卵巢液特性。过熟的雌性三刺鱼的卵巢液比未过熟的雌性少，但干质量更高、蛋白质浓度更高且粘度更低。T和17,20β - P增加了卵巢液的量，17,20β - P提高了卵巢液蛋白质浓度。一维十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）显示，卵巢液含有几种蛋白质，个体差异很大，但各组之间没有一致的差异。一些卵巢液蛋白质似乎与卵中的蛋白质相对应。结果表明，卵巢液的分泌可能受类固醇激素影响，其特性变化与排卵卵的过熟有关。至少在某些方面，卵巢液的变化似乎是过熟的结果而非原因。
