Department of Orthopaedics, Biomechanics and Implant Technology Research Laboratory, Rostock University Medical Center, Rostock, Germany.
Department of Otorhinolaryngology, Rostock University Medical Center, Rostock, Germany.
J Biomed Mater Res B Appl Biomater. 2019 Oct;107(7):2354-2364. doi: 10.1002/jbm.b.34329. Epub 2019 Jan 30.
Devitalization using high hydrostatic pressure (HHP) treatment inactivates cells while matrix structure and biomechanical properties are maintained. Because of strong chondroinductive potential of HHP-devitalized cartilage matrix, it may be used as scaffold for reconstruction of (osteo-)chondral lesions. In this pilot study, we evaluated the feasibility of HHP-devitalized osteochondral tissue to repair osteochondral defects in a rabbit model. Removal and reimplantation of osteochondral plugs were performed in 12 female New Zealand White rabbits. From the knee joint of each animal, osteochondral plugs (diameter = 4 mm; depth = 2.5 mm) were harvested and devitalized by HHP (452 MPa for 10 min). Afterward, the plugs were reimplanted into the respective cavity, from where they were taken. Animals were sacrificed 12 weeks postoperatively and the integration of osteochondral plugs was examined using μ-CT, MRI, and histological staining. Furthermore, revitalization of HHP-treated osteochondral plugs was characterized by gene expression analyses. Macroscopic evaluation of tissue repair at implantation sites of HHP-treated osteochondral plugs showed an adequate defect filling 12 weeks after implantation. Plug margins were hardly detectable indicating successful tissue integration. Additionally, gene expression analyses demonstrated initial revitalization of the HHP-treated tissue 12 weeks postoperatively. Our preliminary data revealed that HHP-treated osteochondral plugs could be used to refill osteochondral defects in the knee joint and promote cell migration into defect site. Data indicated that HHP-treated tissue has the potential to act as functional scaffolds for reconstruction of cartilage defects. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 2354-2364, 2019.
高压（HHP）处理失活通过破坏细胞而保持基质结构和生物力学性能。由于 HHP 失活软骨基质具有很强的软骨诱导潜力，它可以作为（骨）软骨病变重建的支架。在这项初步研究中，我们评估了 HHP 失活骨软骨组织修复兔模型中骨软骨缺损的可行性。在 12 只雌性新西兰白兔中进行了骨软骨栓的切除和再植入。从每个动物的膝关节中，取出并失活骨软骨栓（直径=4mm；深度=2.5mm），通过 HHP（452MPa 处理 10 分钟）。此后，将这些塞子重新植入各自的腔中，然后从腔中取出。术后 12 周处死动物，通过 μ-CT、MRI 和组织学染色检查骨软骨塞的整合情况。此外，通过基因表达分析来表征 HHP 处理的骨软骨塞的再活力化。HHP 处理的骨软骨塞在植入部位的组织修复的宏观评估表明，植入后 12 周有足够的缺陷填充。塞子边缘几乎不可见，表明组织成功整合。此外，基因表达分析表明，HHP 处理组织在术后 12 周有初步的再活力化。我们的初步数据显示，HHP 处理的骨软骨塞可用于填充膝关节的骨软骨缺损，并促进细胞迁移到缺损部位。数据表明，HHP 处理的组织具有作为软骨缺损重建功能支架的潜力。© 2019 Wiley Periodicals, Inc. J 生物材料 Res 部分 B: 应用生物材料 107B: 2354-2364, 2019.
