Malysheva I E, Topchieva L V, Balan O V, Marusenko I M, Barysheva O Y
Institute of Biology of the Karelian Research Centre of the Russian Academy of Sciences, laboratory of genetics; Petrozavodsk, Russia.
Federal State Budgetary Educational Institution of Higher Education «Petrozavodsk State University»; Department of Hospital Therapy, Petrozavodsk, Russia.
Ter Arkh. 2018 May 11;90(5):72-75. doi: 10.26442/terarkh201890572-75.
The aim of this research was studying the level of DROSHA and DICER genes expression in peripheral mononuclear blood cells (PBMC) in rheumatoid arthritis (RA) patients under methotrexate therapy.
82 people (from 45 to 70 years) enrolled in this study were divided into 3 groups (the first one - healthy donors (n=33 median age 49.93±1.87); the second group - rheumatoid arthritis patients without any therapy (n=15; median age 57.28±15.18) and the third group (n=34; median age 60.88±9.02) - rheumatoid arthritis patients who treated at least 4 weeks with methotrexate therapy (10-20 mg/week). The DICER and DROSHA genes expression level was determined by real-time PCR.
The number of DICER gene transcripts in PBMC in rheumatoid arthritis patients without therapy as in RA patients treated with methotrexate was reduced in comparison with the healthy donors (p<0.001 and p>0.05 respectively). The level of DROSHA gene expression in PBMC was not significantly different in all groups enrolled in this study (p>0.05).
Our findings suggest that that the DICER gene expression level in perifheral mononuclear blood cells decreased with the development of rheumatoid arthritis. Methotrexate doesn't influence on the mRNA level of this gene.
本研究旨在探讨甲氨蝶呤治疗的类风湿关节炎（RA）患者外周血单个核细胞（PBMC）中DROSHA和DICER基因的表达水平。
本研究纳入的82例年龄在45至70岁之间的患者被分为3组（第一组——健康供者（n = 33，中位年龄49.93±1.87）；第二组——未接受任何治疗的类风湿关节炎患者（n = 15；中位年龄57.28±15.18）；第三组（n = 34；中位年龄60.88±9.02）——接受甲氨蝶呤治疗（10 - 20mg/周）至少4周的类风湿关节炎患者。通过实时荧光定量PCR测定DICER和DROSHA基因的表达水平。
未接受治疗的类风湿关节炎患者及接受甲氨蝶呤治疗的RA患者PBMC中DICER基因转录本数量与健康供者相比均减少（分别为p<0.001和p>0.05）。本研究纳入的所有组中PBMC中DROSHA基因的表达水平无显著差异（p>0.05）。
我们的研究结果表明，类风湿关节炎患者外周血单个核细胞中DICER基因表达水平随疾病发展而降低。甲氨蝶呤对该基因的mRNA水平无影响。
