Barrueco C, de la Peña E
Instituto de Edafología y Biología Vegetal, Madrid, Spain.
Mutagenesis. 1988 Nov;3(6):467-80. doi: 10.1093/mutage/3.6.467.
A mutagenic evaluation of captan, folpet, captafol, dichlofluanid and related compounds was carried out using the Salmonella/mammalian microsome test using strains TA102 and TA104. These strains contain A-T base pairs at the site of the mutation in contrast to the other Salmonella tester strains that detect mutagens damaging G-C base pairs. In addition, the excision repair system of the TA102 strain is still intact. Captan and folpet were mutagenic in strain TA104, captafol was mutagenic in strain TA102, whereas the remaining test compounds (dichlofluanid, tetrahydrophthalimide and thiozolidine-4-carboxylic acid) were not mutagenic in either strain. In conclusion, we consider it of value to add these two strains to those already used in the Ames test in order to increase confidence in our ability to detect mutagens and to shed further light on their mechanism of action.
使用鼠伤寒沙门氏菌/哺乳动物微粒体试验，以TA102和TA104菌株对克菌丹、灭菌丹、敌菌丹、二氯氟苯胺及其相关化合物进行了致突变性评估。与其他检测损伤G-C碱基对诱变剂的沙门氏菌测试菌株不同，这些菌株在突变位点含有A-T碱基对。此外，TA102菌株的切除修复系统仍然完整。克菌丹和灭菌丹在TA104菌株中具有致突变性，敌菌丹在TA102菌株中具有致突变性，而其余测试化合物（二氯氟苯胺、四氢邻苯二甲酰亚胺和硫代唑烷-4-羧酸）在两种菌株中均无致突变性。总之，我们认为将这两种菌株添加到艾姆斯试验中已使用的菌株中很有价值，以便增强我们检测诱变剂的能力并进一步阐明其作用机制。
