Department of Dental Biomaterials Science, Dental Research Institute and BK21 Plus Program, School of Dentistry, Seoul National University, Seoul, Korea.
Int Endod J. 2019 Jul;52(7):987-998. doi: 10.1111/iej.13088. Epub 2019 Feb 20.
To evaluate the effects of hydrophilic dental resin monomers, triethylene glycol dimethacrylate (TEGDMA) and hydroxyethyl methacrylate (HEMA), on the polarization of a human monocyte cell line (THP-1).
THP-1 cells were treated with resin monomers at noncytotoxic concentrations for 48 h and were analysed for CD86 and CD206 expressions using flow cytometry. The cells were stimulated for polarization in the presence of resin monomers (co-treatment) or after treatment with monomers (pre-treatment). CD86 and CD206 mRNA in co-treated cells was evaluated using quantitative real-time polymerase chain reaction. The release of TNF-α and TGF-β by pre-treated and co-treated cells was assessed using enzyme-linked immunosorbent assay. Morphological changes of macrophages during polarization were observed using bright-field microscopy. One-way analysis of variance was used for statistical analysis.
TEGDMA (1 mmol L ) and HEMA (2 mmol L ) did not induce CD86 and CD206 expressions in THP-1 cells but rather inhibited their expressions in the co-treated cells. The inhibitory effects also appeared at the transcription level. However, the expression of surface markers was not affected by pre-treatment with resin monomers. The release of TNF-α and TGF-β by M1- and M2-stimulated cells, respectively, was suppressed by co-treatment (P < 0.05). Microscopic studies revealed that co-treatment with resin monomers suppressed polarization-associated morphological changes such as cell volume increase.
TEGDMA and HEMA inhibited macrophage polarization to both M1 and M2 at the transcription level, and the inhibitory effects disappeared upon the removal of resin monomers from the cell culture.
评估亲水性牙科树脂单体三乙二醇二甲基丙烯酸酯（TEGDMA）和羟乙基甲基丙烯酸酯（HEMA）对人单核细胞系（THP-1）极化的影响。
将 THP-1 细胞用非细胞毒性浓度的树脂单体处理 48 h，并用流式细胞术分析 CD86 和 CD206 的表达。在存在树脂单体的情况下（共处理）或在用单体处理后（预处理）对细胞进行极化刺激。使用实时定量聚合酶链反应评估共处理细胞中 CD86 和 CD206 的 mRNA 表达。用酶联免疫吸附试验评估预处理和共处理细胞中 TNF-α 和 TGF-β 的释放。用明场显微镜观察极化过程中巨噬细胞的形态变化。采用单因素方差分析进行统计学分析。
TEGDMA（1 mmol/L）和 HEMA（2 mmol/L）不会诱导 THP-1 细胞表达 CD86 和 CD206，但会抑制共处理细胞中的表达。这种抑制作用也出现在转录水平。然而，树脂单体的预处理并不影响表面标志物的表达。共处理抑制了 M1 和 M2 刺激细胞分别释放 TNF-α 和 TGF-β（P<0.05）。显微镜研究表明，共处理树脂单体抑制了与极化相关的形态变化，如细胞体积增大。
TEGDMA 和 HEMA 在转录水平上抑制了巨噬细胞向 M1 和 M2 的极化，并且在从细胞培养物中去除树脂单体后，抑制作用消失。
