Department of Food Function and Labeling, National Institute of Health and Nutrition, National Institutes of Biomedical Innovation, Health and Nutrition, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8636, Japan.
Product Development Laboratory, J-OIL MILLs Inc., 11 Kagetoricho, Totsuka-ku, Yokohama, Kanagawa 245-0064, Japan.
Nutrients. 2019 Jan 30;11(2):297. doi: 10.3390/nu11020297.
The intestinal microbiota may regulate bone metabolism by reducing levels of pro-inflammatory cytokines, and T cells in bone tissues of oestrogen-deficient mice have been reported. Resistant starch (RS) is a type of dietary fibre and results in changes in the composition of the gut microbiota. We evaluated the effects of RS supplemented in diets on intestinal microbial composition, bone mineral density, and inflammatory-gene expression in the colon and bone marrow of ovariectomised (OVX) mice. OVX mice were divided randomly into three groups: OVX control, OVX fed a 20% high amylose corn starch (HAS) diet, and OVX fed a 20% acid-hydrolysed HAS (AH-HAS) diet. HAS and AH-HAS diets contained 6.8% and 12% of RS, respectively. After 6 weeks, treatment with HAS or AH-HAS increased the abundance of spp. in faeces. The AH-HAS diet tended to upregulate mRNA expression of interleukin (IL)-10 in the colon, and downregulate expression of receptor activator of nuclear factor kappa-B ligand and IL-7 receptor genes in the bone marrow of OVX mice. AH-HAS treatment attenuated ovariectomy-induced bone loss. These findings suggest that AH-HAS might change the microbiota and immune status of the bone marrow, resulting in attenuated bone resorption in OVX mice.
肠道微生物群可能通过降低促炎细胞因子的水平来调节骨代谢，并且已经报道雌激素缺乏小鼠的骨组织中的 T 细胞。抗性淀粉（RS）是一种膳食纤维，可导致肠道微生物群组成发生变化。我们评估了在饮食中补充 RS 对去卵巢（OVX）小鼠肠道微生物组成、骨密度以及结肠和骨髓中炎症基因表达的影响。OVX 小鼠随机分为三组：OVX 对照组、喂食 20%高直链淀粉玉米淀粉（HAS）饮食的 OVX 组和喂食 20%酸水解 HAS（AH-HAS）饮食的 OVX 组。HAS 和 AH-HAS 饮食分别含有 6.8%和 12%的 RS。6 周后，HAS 或 AH-HAS 处理增加了粪便中 spp.的丰度。AH-HAS 饮食倾向于上调 OVX 小鼠结肠中白细胞介素（IL）-10 的 mRNA 表达，并下调骨髓中核因子 kappa-B 配体受体激活剂和 IL-7 受体基因的表达。AH-HAS 处理减轻了去卵巢引起的骨丢失。这些发现表明，AH-HAS 可能改变骨髓中的微生物群和免疫状态，从而导致 OVX 小鼠的骨吸收减弱。
