Department of Biochemistry, State University of Maringá, Maringá, Paraná, Brazil.
Department of Biochemistry, State University of Maringá, Maringá, Paraná, Brazil.
Bioresour Technol. 2019 Apr;278:408-423. doi: 10.1016/j.biortech.2019.01.064. Epub 2019 Jan 17.
Ferulic acid and its hydroxycinnamate derivatives represent one of the most abundant forms of low molecular weight phenolic compounds in plant biomass. Feruloyl esterases are part of a microorganism's plant cell wall-degrading enzymatic arsenal responsible for cleaving insoluble wall-bound hydroxycinnamates and soluble cytosolic conjugates. Stimulated by industrial requirements, accelerating scientific discoveries and knowledge transfer, continuous improvement efforts have been made to identify, create and repurposed biocatalysts dedicated to plant biomass conversion and biosynthesis of high-added value molecules. Here we review the basic knowledge and recent advances in biotechnological characteristics and the gene content encoding for feruloyl esterases. Information about several enzymes is systematically organized according to their function, biochemical properties, substrate specificity, and biotechnological applications. This review contributes to further structural, functional, and biotechnological R&D both for obtaining hydroxycinnamates from agricultural by-products as well as for lignocellulose biomass treatments aiming for production of bioethanol and other derivatives of industrial interest.
阿魏酸及其羟基肉桂酸衍生物是植物生物质中低分子量酚类化合物最丰富的形式之一。阿魏酰酯酶是微生物植物细胞壁降解酶系的一部分，负责切割不溶性细胞壁结合的羟基肉桂酸和可溶性细胞质共轭物。受工业需求的刺激，加速科学发现和知识转移，不断努力识别、创造和重新利用专门用于植物生物质转化和高附加值分子生物合成的生物催化剂。在这里，我们回顾了阿魏酰酯酶的生物技术特性和基因含量编码的基础知识和最新进展。根据酶的功能、生化特性、底物特异性和生物技术应用，对几种酶的信息进行了系统的组织。本综述有助于进一步进行结构、功能和生物技术研发，以从农业副产物中获得羟基肉桂酸，以及处理木质纤维素生物质，以生产生物乙醇和其他具有工业价值的衍生物。
