Department of Chemistry, University of Manitoba, 144 Dysart Road, Winnipeg, Manitoba, R3T 2N2, Canada.
Centre for Oil and Gas Research and Development (COGRAD), 144 Dysart Road, Winnipeg, MB, R3T 2N2, Canada.
Sci Rep. 2019 Jan 31;9(1):1058. doi: 10.1038/s41598-018-37323-x.
Polycyclic aromatic hydrocarbons (PAHs) are toxic, mutagenic and among the most damaging chemical compounds with regard to living organisms. Because of their persistence and wide distribution removal from the environment is an important challenge. Here we report a new Nano container matrix based on the deep sea archaea-derived RHCC-Nanotube (RHCC-NT), which rapidly and preferentially binds low molecular weight PAHs. Under controlled-laboratory conditions and using fluorescence spectroscopy in combination with X-ray crystallography and MD simulations, we quantified the real-time binding of low molecular weight PAHs (2-4 rings) to our substrate. Binding coefficients ranged from 5.4 ± 1.6 (fluorene) to 32 ± 7.0 μM (acenaphthylene) and a binding capacity of 85 pmoles PAH per mg RHCC-NT, or 2.12 μmoles in a standard 25 mg sampler. The uptake rate of pyrene was calculated to be 1.59 nmol/hr∙mol RHCC-NT (at 10 C). Our results clearly show that RHCC-NT is uniquely suited as a monitoring matrix for low molecular weight PAHs.
多环芳烃（PAHs）是有毒的、致突变的，并且是对生物体危害最大的化学物质之一。由于它们的持久性和广泛分布，从环境中去除它们是一个重要的挑战。在这里，我们报告了一种基于深海古菌衍生的 RHCC-纳米管（RHCC-NT）的新型纳米容器基质，它可以快速且优先结合低分子量 PAHs。在受控实验室条件下，我们使用荧光光谱学结合 X 射线晶体学和 MD 模拟，实时定量了低分子量 PAHs（2-4 环）与我们的基质的结合。结合系数范围从 5.4 ± 1.6（芴）到 32 ± 7.0 μM（苊烯），每毫克 RHCC-NT 的结合容量为 85 pmoles PAH，或在标准 25 mg 采样器中为 2.12 μmoles。计算得出的蒽的摄取速率为 1.59 nmol/hr·mol RHCC-NT（在 10°C 时）。我们的结果清楚地表明，RHCC-NT 非常适合作为低分子量 PAHs 的监测基质。
