Zhou Yue, Dai Hong, Long Juan, Kang Xin-Guo, He Chun-Jing
Department of Pain, Guizhou Provincial People's Hospital, Guiyang 550002, China,
Department of Neurology, Guizhou Provincial People's Hospital, Guiyang 550002, China.
J Pain Res. 2019 Jan 17;12:387-394. doi: 10.2147/JPR.S177585. eCollection 2019.
The aim of this study was to observe the effects of extracorporeal shock waves (ECSWs) on neuralgia in diabetic rats.
Diabetic neuralgia model was established in rats via injection of streptozotocin. The rats were divided into diabetic neuralgia group (Group A, n=6) and ECSW treatment group (Group B, n=6). Another six rats were taken as control group (Group C, n=6). The mechanical withdrawal threshold (MWT) and thermal withdrawal latencies (TWLs) were measured at specific points throughout the experiment, and the sciatic nerve was bluntly severed under anesthesia after the last measurement. The protein expressions of Sphk1 and TNF-α were detected by Western blot, and the mRNA expressions of Sphk1 and TNF-α were detected by reverse transcription PCR. The structure of the sciatic nerve was observed by electron microscopy.
Compared with Group C, MWT and TWLs were decreased significantly in Groups A and B (< 0.05). The protein expressions of TNF-α and Sphk1 in Groups A and B were both significantly higher than those in Group C (<0.05), with higher expression in Group A than in Group B (<0.05). The mRNA expressions of TNF-α and Sphk1 were similar. Electron microscopy showed the intact structure of the myelin sheaths of the sciatic nerve fibers in Group C, whereas the structure of the nerve fibers was damaged, with a large number of vacuoles in the myelin sheath in Group A. In Group B, the vacuoles were occasionally formed on the sciatic nerve myelin sheath, with more compact and tidy layer arrangement compared with Group A.
ECSWs can relieve neuralgia in diabetic rats. Sphk1 and TNF-α may be involved in the occurrence and development of diabetic peripheral neuralgia.
本研究旨在观察体外冲击波（ECSWs）对糖尿病大鼠神经痛的影响。
通过注射链脲佐菌素建立大鼠糖尿病神经痛模型。将大鼠分为糖尿病神经痛组（A组，n = 6）和ECSW治疗组（B组，n = 6）。另取6只大鼠作为对照组（C组，n = 6）。在整个实验过程中的特定时间点测量机械性撤针阈值（MWT）和热缩足潜伏期（TWLs），最后一次测量后在麻醉下钝性切断坐骨神经。采用蛋白质免疫印迹法检测Sphk1和TNF-α的蛋白表达，采用逆转录聚合酶链反应检测Sphk1和TNF-α的mRNA表达。通过电子显微镜观察坐骨神经的结构。
与C组相比，A组和B组的MWT和TWLs均显著降低（<0.05）。A组和B组中TNF-α和Sphk1的蛋白表达均显著高于C组（<0.05），且A组表达高于B组（<0.05）。TNF-α和Sphk1的mRNA表达情况相似。电子显微镜显示C组坐骨神经纤维髓鞘结构完整，而A组神经纤维结构受损，髓鞘内有大量空泡。B组坐骨神经髓鞘偶尔有空泡形成，与A组相比，层排列更紧密、整齐。
ECSWs可缓解糖尿病大鼠的神经痛。Sphk1和TNF-α可能参与糖尿病周围神经痛的发生和发展。
