Cellular Reprogramming and Embryo Biotechnology Laboratory, Dental Research Institute, BK21, Seoul National University School of Dentistry, Seoul 08826, Korea.
Biomedical Research Institute, NeoRegen Biotech Co., Ltd., Gyeonggi-do 16614, Korea.
Int J Mol Sci. 2019 Jan 31;20(3):624. doi: 10.3390/ijms20030624.
Hyaline cartilage is a tissue of very low regenerative capacity because of its histology and limited nutrient supply. Cell-based therapies have been spotlighted in the regeneration of damaged cartilage. Dental pulp stem cells (DPSCs) are multipotent and are easily accessible for therapeutic purposes. In human gastrointestinal tracts, is a naturally occurring commensal species of lactic acid bacteria. In this work, the human DPSCs were differentiated into chondrocytes using a chondrogenic differentiation medium with or without L-15 extract. We observed that chondrogenic differentiation improved in an L-15 extract (L-15)-treated DPSC group via evaluation of chondrogenic-marker mRNA expression levels. In particular, we found that L-15 treatment promoted early-stage DPSC differentiation. Cells treated with L-15 were inhibited at later stages and were less likely to transform into hypertrophic chondrocytes. In L-15-treated groups, the total amount of cartilage extracellular matrix increased during the differentiation process. These results suggest that L-15 promotes chondrogenic differentiation, and that L-15 may be used for cartilage repair or cartilage health supplements. To our knowledge, this is the first report demonstrating the beneficial effect of L-15 treatment on chondrogenic differentiation.
透明软骨组织的再生能力非常低，这与其组织学特性和有限的营养供应有关。基于细胞的疗法已成为受损软骨再生的研究热点。牙髓干细胞（DPSCs）具有多能性，并且易于用于治疗目的。在人类胃肠道中，是一种天然存在的乳酸菌共生种。在这项工作中，使用含或不含 L-15 提取物的软骨细胞分化培养基将人 DPSCs 分化为软骨细胞。我们通过评估软骨形成标志物 mRNA 表达水平发现，在添加 L-15 提取物（L-15）的 DPSC 组中，软骨细胞分化得到了改善。特别是，我们发现 L-15 处理促进了早期 DPSC 分化。在后期处理 L-15 的细胞受到抑制，并且不太可能转化为肥大软骨细胞。在 L-15 处理组中，软骨细胞外基质的总量在分化过程中增加。这些结果表明 L-15 可促进软骨细胞分化，并且 L-15 可用于软骨修复或软骨健康补充剂。据我们所知，这是第一项证明 L-15 处理对软骨细胞分化有益的报告。
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