Secretagogin Purification and Quality Control Strategies for Biophysical and Cell Biological Studies.

Secretagogin (SCGN) has recently gained attention due to its modulatory effect on insulin/CRH secretion and function. However, a large pool of speculated SCGN functions remains unexplored. A major deficiency is the lack of knowledge about the biological functions of extracellular SCGN. We here describe convenient methods for the scalable production of His-tagged and untagged mouse SCGN. The protocol is optimized to remove endotoxins, and thus the protein is suited for biological applications such as cell culture treatment or animal injections. We also outline expedient methods to check the purity of SCGN preparation for biological applications.