Sreejit Gopalkrishna, Nooti Sunil Kiran, Athmanathan Baskaran, Nagareddy Prabhakara Reddy
Department of Pathology, University of Alabama, Birmingham, AL, USA.
Methods Mol Biol. 2019;1929:739-754. doi: 10.1007/978-1-4939-9030-6_46.
S100A8/A9 represents a novel biomarker and therapeutic target in sterile inflammatory diseases. Among the various S100 proteins, S100A8 and S100A9 have been shown to be the most important of all the damage-associated molecular pattern (DAMP) proteins in sterile inflammatory conditions such as diabetes, cardiovascular disease, autoimmune disorders, etc. We present here methods to quantify S100A8/A9 expression in various tissues in mouse models of myocardial infarction (MI) using flow cytometry (FC), immunofluorescence, quantitative real-time polymerase chain reaction (q-RT-PCR), and enzyme-linked immunosorbent assays (ELISA).
S100A8/A9是无菌性炎症疾病中的一种新型生物标志物和治疗靶点。在各种S100蛋白中，S100A8和S100A9已被证明是糖尿病、心血管疾病、自身免疫性疾病等无菌性炎症状态下所有损伤相关分子模式（DAMP）蛋白中最重要的。我们在此介绍了使用流式细胞术（FC）、免疫荧光、定量实时聚合酶链反应（q-RT-PCR）和酶联免疫吸附测定（ELISA）来量化心肌梗死（MI）小鼠模型各种组织中S100A8/A9表达的方法。
