Laboratory for Reproductive Immunology, Hospital and Institute of Obstetrics and Gynecology, Shanghai Medical School, Fudan University, Shanghai, China.
Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai, China.
Int J Gynaecol Obstet. 2019 Apr;145(1):12-17. doi: 10.1002/ijgo.12779. Epub 2019 Feb 22.
To evaluate the association between CD16 monocytes in peripheral blood and the clinical features and short-term therapeutic effects of polycystic ovary syndrome (PCOS).
This prospective cross-sectional study included women diagnosed with PCOS at a University Hospital in Shanghai, China, between June 4 and November 28, 2016. Patients received Diane-35, metformin, or both combined for 3 months. We collected anthropometric measures and used flow cytometry to detect CD16 monocytes.
The final analysis included 70 patients: 18 in the Diane-35 group, 30 in the metformin group, and 22 in the Diane-35 plus metformin group. The control group comprised 60 women without PCOS. The proportion of CD16 monocytes was significantly higher in patients with PCOS than in those with no PCOS (16.05% vs 10.73%; P=0.001). The proportion differed significantly between patients with and those without hyperandrogenism (13.12% vs 17.30%; P=0.002) and showed moderate accuracy in diagnosing hyperandrogenism before treatment. We noted a decrease in monocytes post-treatment in patients given metformin and Diane-35 plus metformin.
The proportion of CD16 monocytes was most significantly associated with hyperandrogenism before treatment. Our findings suggest that the proportion of CD16 monocytes in peripheral blood might be related to the inflammatory condition of PCOS.
评估外周血 CD16 单核细胞与多囊卵巢综合征（PCOS）的临床特征和短期治疗效果之间的关系。
本前瞻性横断面研究纳入了 2016 年 6 月 4 日至 11 月 28 日期间在中国上海一家大学医院诊断为 PCOS 的女性患者。患者接受达英-35、二甲双胍或两者联合治疗 3 个月。我们收集了人体测量指标，并使用流式细胞术检测 CD16 单核细胞。
最终分析纳入了 70 例患者：达英-35 组 18 例，二甲双胍组 30 例，达英-35 加二甲双胍组 22 例。对照组包括 60 例无 PCOS 的女性。PCOS 患者的 CD16 单核细胞比例明显高于无 PCOS 患者（16.05%比 10.73%；P=0.001）。患有高雄激素血症的患者与不患有高雄激素血症的患者之间的比例差异显著（13.12%比 17.30%；P=0.002），并且在治疗前对高雄激素血症具有中等准确性的诊断。我们注意到接受二甲双胍和达英-35 加二甲双胍治疗的患者治疗后单核细胞减少。
CD16 单核细胞的比例与治疗前的高雄激素血症最显著相关。我们的研究结果表明，外周血 CD16 单核细胞的比例可能与 PCOS 的炎症状态有关。
