Laboratory of Developmental Biology, Department of Cellular and Molecular Biology, Faculty of Biology and Environmental Protection, Nicolaus Copernicus University in Toruń, Lwowska 1, 87-100 Toruń, Poland.
Laboratory of Molecular and Isotope Methods, Department of Cellular and Molecular Biology, Faculty of Biology and Environmental Protection, Nicolaus Copernicus University in Toruń, Lwowska 1, 87-100 Toruń, Poland.
Mol Phylogenet Evol. 2019 May;134:99-110. doi: 10.1016/j.ympev.2019.01.014. Epub 2019 Jan 31.
Calreticulin (CRT) is an multifunctional resident endoplasmic reticulum (ER) luminal protein implicated in regulating a variety of cellular processes, including Ca storage/mobilization and protein folding. These multiple functions may be carried out by different CRT genes and protein isoforms. The plant CRT family consist of three genes: CRT1 and CRT2 classified in the common subclass (CRT1/2), and CRT3. These genes are highly conserved during evolution and encode three different protein products (CRT1, 2 and 3). The aim of the current study was to conduct a comparative analysis and sequence-based classification of the plant CRT genes. We used nucleotide and amino acid sequences to phylogenetically cluster the genes and examine potential glycosylation patterns. Additionally, we analyzed phylogenetic relationships within the CRT subclasses. Finally, we analyzed intraspecific CRT duplication events among mono- and dicotyledon species. Our results confirm that each of the CRT genes exist in multiple copies in plant genomes, and that CRT gene duplication is a widespread process in plants.
钙网蛋白（Calreticulin，CRT）是一种多功能内质网（Endoplasmic Reticulum，ER）腔蛋白，参与调节多种细胞过程，包括 Ca 储存/动员和蛋白质折叠。这些多种功能可能由不同的 CRT 基因和蛋白同工型执行。植物 CRT 家族由三个基因组成：CRT1 和 CRT2 归为常见亚类（CRT1/2），以及 CRT3。这些基因在进化过程中高度保守，编码三种不同的蛋白产物（CRT1、2 和 3）。本研究旨在对植物 CRT 基因进行比较分析和基于序列的分类。我们使用核苷酸和氨基酸序列对基因进行系统发育聚类，并研究潜在的糖基化模式。此外，我们还分析了 CRT 亚类内的系统发育关系。最后，我们分析了单子叶和双子叶植物种内的 CRT 重复事件。我们的结果证实，每个 CRT 基因在植物基因组中都存在多个拷贝，并且 CRT 基因复制是植物中广泛存在的过程。
