Kusuhara Yoshito, Daizumoto Kei, Kawai Kaichi, Hirayama Kento, Kowada Minoru, Shintani Terumichi, Fukuhara Yayoi, Dondoo Tsogt-Ochir, Ozaki Keisuke, Tsuda Megumi, Fukawa Tomoya, Nakatsuji Hiroyoshi, Bando Yoshimi, Uehara Hisanori, Fukumori Tomoharu, Takahashi Masayuki, Kanayama Hiro-Omi
Department of Urology, Tokushima University Graduate School of Biomedical Sciences, Tokushima, Japan.
Department of Urology, Tokushima University Graduate School of Biomedical Sciences, Tokushima, Japan
Anticancer Res. 2019 Feb;39(2):703-711. doi: 10.21873/anticanres.13166.
BACKGROUND/AIM: The aim of this study was to elucidate the relationship between the progression of bladder cancer (BCa) and TLR4 expression.
The relationship between TLR4 expression and prognosis of BCa patients was analyzed using a publicly available database and immunohistochemical staining of clinical samples. The effect of TLR4 knockdown was also examined on the invasive capabilities of BCa cells. Finally, to investigate the biological function of TLR4, the gene expression profile of TLR4-depleted BCa cells was analyzed by microarray analysis.
Expression of TLR4 was inversely associated with prognosis of patients with invasive BCa, and depletion of TLR4 significantly enhanced the invasive capability of BCa cells. Gene expression profiling revealed that depletion of TLR4 led to high expression of epithelial differentiation genes. Furthermore, expression of TLR4 was found to be extremely low in areas of squamous differentiation.
Low TLR4 expression was correlated with tumor progression.
背景/目的：本研究旨在阐明膀胱癌（BCa）进展与Toll样受体4（TLR4）表达之间的关系。
利用公开数据库及临床样本免疫组化染色分析TLR4表达与BCa患者预后的关系。还检测了TLR4基因敲低对BCa细胞侵袭能力的影响。最后，通过基因芯片分析来研究TLR4基因敲低的BCa细胞的基因表达谱，以探讨TLR4的生物学功能。
TLR4表达与浸润性BCa患者的预后呈负相关，TLR4基因敲低显著增强了BCa细胞的侵袭能力。基因表达谱显示，TLR4基因敲低导致上皮分化基因高表达。此外，在鳞状分化区域发现TLR4表达极低。
TLR4低表达与肿瘤进展相关。
