Institute of Chemical Industry of Forest Products, CAF, Nanjing 210042, Jiangsu, China; Key Laboratory of Biomass Energy and Material, Nanjing 210042, Jiangsu, China; National Engineering Laboratory for Biomass Chemical Utilization, Nanjing 210042, Jiangsu, China; Key and Open Laboratory on Forest Chemical Engineering, SFA, Nanjing 210042, Jiangsu, China.
Institute of Chemical Industry of Forest Products, CAF, Nanjing 210042, Jiangsu, China; Key Laboratory of Biomass Energy and Material, Nanjing 210042, Jiangsu, China; National Engineering Laboratory for Biomass Chemical Utilization, Nanjing 210042, Jiangsu, China; Key and Open Laboratory on Forest Chemical Engineering, SFA, Nanjing 210042, Jiangsu, China; Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210042, Jiangsu, China.
J Chromatogr A. 2019 Apr 12;1590:58-64. doi: 10.1016/j.chroma.2019.01.047. Epub 2019 Jan 21.
Polyprenols extracted from Ginkgo biloba leaves is a kinds of unsaturated compound containing double bonds. Traditionally, the separation methods for the polyprenols are lack of selectivity and their separation efficiency are low. We synthesized two kinds of functional nano-silica containing silver ions materials (AgTCM and AgTCN) which have selectivity for unsaturated compounds to separate Ginkgo biloba leaves polyprenols for the first time. AgTCN displays exceptionally high selectivity for polyprenols and high stability under extended heat and light exposure, while silver is virtually immobile during solvent elution. Importantly, the exceptional stability of AgTCN gives rise to much higher polyprenols recovery than conventional silica gel during the chromatographic elution. In addition, we found that the adsorption of polyprenols onto the AgTCN conforms to pseudo-second-order kinetic model and AgTCN has strong affinity with polyprenols by analyzing Langmuir, Freundlich, Temkin-Pyzhev, and Dubinin-Radushkevich isotherms. The calculation results of thermodynamic parameters demonstrate that decrease of temperature in favor of increasing the adsorbing capacity of polyprenols onto the AgTCN, and the adsorption process of which is exothermic reaction. Our results pave the way for the novel separation methods of polyprenols from Ginkgo biloba leaves.
银杏叶中提取的多萜醇是一种含有双键的不饱和化合物。传统的多萜醇分离方法缺乏选择性，分离效率低。我们首次合成了两种对不饱和化合物具有选择性的含银离子功能纳米二氧化硅材料（AgTCM 和 AgTCN），用于分离银杏叶多萜醇。AgTCN 对多萜醇表现出异常高的选择性和在长时间受热和光照暴露下的高稳定性，而在溶剂洗脱过程中银几乎不移动。重要的是，AgTCN 的异常稳定性导致在色谱洗脱过程中多萜醇的回收率比传统硅胶高得多。此外，我们发现多萜醇在 AgTCN 上的吸附符合伪二级动力学模型，并且通过分析 Langmuir、Freundlich、Temkin-Pyzhev 和 Dubinin-Radushkevich 等温线，AgTCN 与多萜醇具有很强的亲和力。热力学参数的计算结果表明，降低温度有利于增加多萜醇在 AgTCN 上的吸附容量，且吸附过程为放热反应。我们的研究结果为银杏叶多萜醇的新型分离方法奠定了基础。
