Carroll Courtney, Olsen Kyle D, Ricks Nathan J, Dill-McFarland Kimberly A, Suen Garret, Robinson Todd F, Chaston John M
Department of Plant and Wildlife Sciences, Brigham Young University, Provo, UT, United States.
Department of Bacteriology, University of Wisconsin-Madison, Madison, WI, United States.
Front Microbiol. 2019 Jan 18;9:3334. doi: 10.3389/fmicb.2018.03334. eCollection 2018.
Gut -associated microbes ('gut microbiota') impact the nutrition of their hosts, especially in ruminants and pseudoruminants that consume high-cellulose diets. Examples include the pseudoruminant alpaca. To better understand how body site and diet influence the alpaca microbiota, we performed three 16S rRNA gene surveys. First, we surveyed the compartment 1 (C1), duodenum, jejunum, ileum, cecum, and large intestine (LI) of alpacas fed a grass hay (GH; tall fescue) or alfalfa hay (AH) diet for 30 days. Second, we performed a C1 survey of alpacas fed a series of 2-week mixed grass hay (MGH) diets supplemented with ∼25% dry weight barley, quinoa, amaranth, or soybean meal. Third, we examined the microbial differences of alpacas with normal versus poor body condition. Samples from GH- and AH-fed alpacas grouped by diet and body site but none of the four supplements significantly altered C1 microbiota composition, relative to each other, and none of the OTUs were differentially abundant between alpacas with normal versus poor body conditions. Taken together, the findings of a diet- and body-site specific alpaca microbiota are consistent with previous findings in ruminants and other mammals, but we provide no evidence to link changes in alpaca body condition with variation in microbiota relative abundance or identity.
肠道相关微生物（“肠道微生物群”）会影响宿主的营养状况，尤其是在食用高纤维素日粮的反刍动物和伪反刍动物中。例子包括伪反刍动物羊驼。为了更好地了解身体部位和日粮如何影响羊驼微生物群，我们进行了三项16S rRNA基因调查。首先，我们调查了喂食禾本科干草（GH；高羊茅）或苜蓿干草（AH）日粮30天的羊驼的第1隔室（C1）、十二指肠、空肠、回肠、盲肠和大肠（LI）。其次，我们对喂食一系列为期2周的混合禾本科干草（MGH）日粮（添加约25%干重的大麦、藜麦、苋属植物或豆粕）的羊驼进行了C1调查。第三，我们研究了身体状况正常与较差的羊驼之间的微生物差异。来自喂食GH和AH的羊驼的样本按日粮和身体部位分组，但相对于彼此，四种补充剂均未显著改变C1微生物群组成，并且在身体状况正常与较差的羊驼之间，没有OTU的丰度存在差异。综上所述，羊驼微生物群具有日粮和身体部位特异性的研究结果与反刍动物和其他哺乳动物之前的研究结果一致，但我们没有提供证据表明羊驼身体状况的变化与微生物群相对丰度或种类的变化有关。
