Khalili Sara, Mohebali Mehdi, Ebrahimzadeh Elaheh, Shayan Ebrahimzadeh, Mohammadi-Yeganeh Samira, Moosazadeh Moghaddam Mehrdad, Elikaee Samira, Akhoundi Behnaz, Sharifi-Yazdi Mohammad Kazem
Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Vet Res Forum. 2018 Fall;9(4):323-328. doi: 10.30466/vrf.2018.33107. Epub 2018 Dec 15.
Zoonotic cutaneous leishmaniasis caused by is a most common type of vector-borne disease in Iran. The pentavalent antimonial drugs have been used in the treatment of cutaneous leishmaniasis for a long time, but drug resistance and some of serious side effects have been reported. Thus, discovery and development of new therapeutic candidates are needed. The CM11 peptide is one of these peptides that its anti-bacterial activity has been proven. This peptide is a short cecropin-melittin hybrid peptide obtained through a sequence combination approach. The aim of this study was to evaluate anti-leishmanial activity of CM11 peptide against amastigote forms of . In this study, amastigote forms of Iranian strain of (MRHO/IR/75/ER) were cultured in the presence of different concentrations of meglumine antimoniate (Glucantime) to find the most appropriate concentration of Glucantime against amastigotes. Then, the anti-leishmanial activities of various concentrations of CM11 peptide (8, 16, 32 and 64 µM) were evaluated for 24, 48 and 72 hr by DAPI staining. In addition, MTT assay was used to determine the cytotoxic effects of CM11 peptide on murine fibroblast cell line. The results showed that CM11 peptide has antimicrobial activity against Iranian isolate of in the laboratory conditions. It seems that the CM11 peptide has significant potential to be used as a new anti-leishmanial agent.
由[病原体名称未给出]引起的人兽共患皮肤利什曼病是伊朗最常见的一种媒介传播疾病。五价锑药物长期以来一直用于治疗皮肤利什曼病，但已报道出现耐药性和一些严重的副作用。因此，需要发现和开发新的治疗候选药物。CM11肽是已被证实具有抗菌活性的此类肽之一。该肽是一种通过序列组合方法获得的短的天蚕素 - 蜂毒肽杂合肽。本研究的目的是评估CM11肽对[病原体名称未给出]无鞭毛体形式的抗利什曼活性。在本研究中，将伊朗[病原体名称未给出]菌株（MRHO/IR/75/ER）的无鞭毛体形式在不同浓度的葡甲胺锑酸盐（葡醛酯）存在下培养，以找到针对[病原体名称未给出]无鞭毛体的最适宜的葡醛酯浓度。然后，通过DAPI染色评估不同浓度（8、16、32和64 μM）的CM11肽在24、48和72小时的抗利什曼活性。此外，使用MTT法测定CM11肽对小鼠成纤维细胞系的细胞毒性作用。结果表明，在实验室条件下，CM11肽对伊朗分离的[病原体名称未给出]具有抗菌活性。CM11肽似乎有很大潜力用作一种新的抗利什曼病药物。
