全基因组鸟枪法测序及丁酸梭菌益生菌株UBCB 70的特性分析以评估其安全性。
Whole-Genome Shotgun Sequencing and Characterization of Probiotic Strain Clostridium butyricum UBCB 70 To Assess Its Safety.
作者信息
Sulthana Ayesha, Thorramamidi Archana, Lakshmi Suvarna G, Madempudi Ratna Sudha
机构信息
Center for Research and Development, Unique Biotech Limited, Hyderabad, Telangana, India.
出版信息
Microbiol Resour Announc. 2019 Jan 31;8(5). doi: 10.1128/MRA.01732-18. eCollection 2019 Jan.
Abstract
Clostridium butyricum is a strictly anaerobic, butyric acid-producing, Gram-positive, spore-forming bacillus that is commonly present in the gut of humans. The complete genome sequence of Clostridium butyricum UBCB 70 was studied to evaluate the presence of antibiotic-resistant and clostridium toxin genes. Here, we announce the draft genome sequence of Clostridium butyricum UBCB 70, isolated from healthy human feces at Unique Biotech Limited, Hyderabad, Telangana, India.
摘要
丁酸梭菌是一种严格厌氧、产丁酸、革兰氏阳性、形成芽孢的杆菌，常见于人类肠道中。对丁酸梭菌UBCB 70的全基因组序列进行了研究，以评估抗生素抗性基因和梭菌毒素基因的存在情况。在此，我们公布从印度特伦甘纳邦海得拉巴市Unique Biotech Limited公司健康人类粪便中分离出的丁酸梭菌UBCB 70的基因组草图序列。
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本文引用的文献
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2
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Nucleic Acids Res. 2016 Jan 4;44(D1):D694-7. doi: 10.1093/nar/gkv1239. Epub 2015 Nov 17.
3
In silico detection and typing of plasmids using PlasmidFinder and plasmid multilocus sequence typing.使用质粒查找工具（PlasmidFinder）和质粒多位点序列分型进行质粒的电子检测和分型
Antimicrob Agents Chemother. 2014 Jul;58(7):3895-903. doi: 10.1128/AAC.02412-14. Epub 2014 Apr 28.
4
A comprehensive evaluation of assembly scaffolding tools.装配脚手架工具的综合评估。
Genome Biol. 2014 Mar 3;15(3):R42. doi: 10.1186/gb-2014-15-3-r42.
5
SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing.SPAdes：一种新的基因组组装算法及其在单细胞测序中的应用
J Comput Biol. 2012 May;19(5):455-77. doi: 10.1089/cmb.2012.0021. Epub 2012 Apr 16.
6
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7
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8
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