Universidad Autónoma de Madrid, Department of Molecular Biology, Madrid 28049, Spain; Federal University of Pernambuco, Department of Civil and Environmental Engineering, Laboratory of Environmental Sanitation, Recife, PE 50740-530, Brazil.
Universidad Autónoma de Madrid, Department of Molecular Biology, Madrid 28049, Spain.
Bioresour Technol. 2019 May;279:140-148. doi: 10.1016/j.biortech.2019.01.125. Epub 2019 Jan 26.
The 1,3-propanediol (1,3-PDO) yield and productivity from glycerol were studied over a 155-day period. A UASB reactor that also contained silicone support for biomass attachment was used to evaluate the optimal operational conditions and microbiota development. The highest average 1,3-PDO yield was 0.54 and 0.48 mol.mol-gly when reactor pH was 5.0-5.5 and the applied loading rate was 18 and 20 g-gly.L.d using the pure and crude substrate, respectively. The productivity was close to 7.5 g.L.d for both substrates; therefore, the direct use of crude glycerol can be valorized in practice. Clostridium was the predominant genus for 1,3-PDO production and C. pasteurianum was dominant in the biofilm. Using crude glycerol, C. beijerinckii dropped strongly; some Clostridium population was then replaced by Klebsiella pneumoniae and Lactobacillus spp. The good process performance and the advances in the microbiota knowledge are steps forward to obtain a more cost-effective system in practice.
在 155 天的时间里，研究了甘油转化为 1,3-丙二醇（1,3-PDO）的产率和生产力。使用含有硅酮生物质附着支撑物的 UASB 反应器来评估最佳操作条件和微生物群落的发展。当反应器 pH 值为 5.0-5.5 且纯底物和粗底物的应用加载率分别为 18 和 20 g-gly.L.d 时，获得的最高平均 1,3-PDO 产率分别为 0.54 和 0.48 mol.mol-甘油。对于这两种底物，生产力均接近 7.5 g.L.d；因此，粗甘油可以直接用于实际应用中。产 1,3-PDO 的主要属是梭菌属，生物膜中的优势菌是丙酮丁醇梭菌。使用粗甘油时，拜氏梭菌急剧下降；一些梭菌种群随后被肺炎克雷伯菌和乳杆菌属取代。良好的工艺性能和微生物群落知识的进步是在实践中获得更具成本效益的系统的重要步骤。
