Department of Food Engineering (DEA), Faculty of Food Engineering (FEA), University of Campinas (UNICAMP), Rua Monteiro Lobato, 80; Campinas-SP, CEP: 13083-862, Brazil.
Centre of Biological Engineering (CEB), University of Minho (UMINHO), Campus Gualtar, 4710 Braga, Portugal.
Food Res Int. 2019 Feb;116:628-636. doi: 10.1016/j.foodres.2018.08.087. Epub 2018 Aug 28.
Sodium caseinate (NaCAS) is widely used in the food industry to provide nutritional and functional benefits. This work deals with the effects of applying moderate electric fields (MEF) of different intensity - ranging from 2 V·cm to 17 V·cm - on the physical and functional properties of NaCAS solutions during Ohmic heating (OH) at 95 °C. Self-standing gels were produced regardless the heating technique applied (i.e. conventional or OH), and these gels were much more prone to physical rupture when compared with the ones produced from unheated NaCAS. Interestingly, OH treatment formed gels with lower values of strain at rupture and water holding capacity than unheated samples; this pattern was not observed for gels obtained through the conventional heating treatment (at 0 V·cm). These effects may be linked with disturbances of the distribution of random coil structures and enhanced solubility of NaCAS at its isoelectric point, reducing aggregation and impairing the development of a more compact protein network. Results show that OH presents potential to be used as volumetric heating tool for NaCAS solubilization and for the production of distinctive acidified systems.
酪蛋白酸钠(NaCAS)在食品工业中被广泛应用,以提供营养和功能性益处。本工作研究了在 95°C 欧姆加热(OH)过程中,应用不同强度的适度电场(MEF)- 范围为 2V·cm 至 17V·cm - 对 NaCAS 溶液物理和功能特性的影响。无论应用哪种加热技术(即常规加热或 OH 加热),都可以制备出自立凝胶,与未经加热的 NaCAS 相比,这些凝胶更容易发生物理破裂。有趣的是,OH 处理形成的凝胶在断裂时的应变量和持水力值低于未经加热的样品;而通过常规加热处理(在 0V·cm 下)获得的凝胶则没有观察到这种模式。这些影响可能与无规卷曲结构分布的干扰以及 NaCAS 在等电点下的溶解度增加有关,这会减少聚集并损害更紧凑的蛋白质网络的发展。结果表明,OH 具有作为 NaCAS 溶解和生产独特酸化系统的体积加热工具的潜力。