Hampel A, Tritz R
Methods Enzymol. 1988;166:260-5. doi: 10.1016/s0076-6879(88)66035-6.
The methodologies described in this chapter allow the reproducible preparation of native high-molecular-weight synthetase complexes of leuRL. These complexes have the ability to preferentially utilize extracellular leucine immediately upon transport and are likely the forms of the enzyme most important in the utilization of leucine for protein synthesis.
本章所述方法能够可重复地制备亮氨酰-tRNA合成酶(leuRL)的天然高分子量合成酶复合物。这些复合物在转运后能够优先利用细胞外亮氨酸,并且可能是亮氨酸用于蛋白质合成过程中最重要的酶形式。