Measurement of branched-chain alpha-keto acid dehydrogenase flux rates in perfused heart and liver.
作者信息
Lapointe D S, Hildebrandt E, Buxton D B, Patel T B, Waymack P P, Olson M S
出版信息
Methods Enzymol. 1988;166:484-97. doi: 10.1016/s0076-6879(88)66062-9.
PMID:3071723
Abstract
The methods described here represent a flexible set of procedures for investigating the metabolism of the branched-chain alpha-keto acids and other substances in perfused organs, notably the rat heart and liver. These procedures have been used to investigate many aspects of the metabolism of the branched-chain alpha-keto acids not discussed here, such as the effects on branched-chain alpha-keto acid metabolism by exposure to alpha-adrenergic agents, by inhibition of the monocarboxylate translocator, and by the coinfusion of other metabolites.
摘要
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