Lapointe D S, Hildebrandt E, Buxton D B, Patel T B, Waymack P P, Olson M S
Methods Enzymol. 1988;166:484-97. doi: 10.1016/s0076-6879(88)66062-9.
The methods described here represent a flexible set of procedures for investigating the metabolism of the branched-chain alpha-keto acids and other substances in perfused organs, notably the rat heart and liver. These procedures have been used to investigate many aspects of the metabolism of the branched-chain alpha-keto acids not discussed here, such as the effects on branched-chain alpha-keto acid metabolism by exposure to alpha-adrenergic agents, by inhibition of the monocarboxylate translocator, and by the coinfusion of other metabolites.
