Reproductive Medicine Center, Renmin Hospital of Wuhan University, Hubei Clinic Research Center for Assisted Reproductive Technology and Embryonic Development, Wuhan University, Wuhan, Hubei 430060, P.R. China.
Mol Med Rep. 2019 Apr;19(4):2620-2626. doi: 10.3892/mmr.2019.9898. Epub 2019 Jan 28.
A disintegrin and metalloproteinase with thrombospondin motifs 7 (ADAMTS‑7) has been revealed to serve an important role in inflammation‑associated diseases. However, the role of ADAMTS‑7 in spontaneous abortion (SA) remains unclear. In the present study, human and mouse decidual tissues were used to detect the expression of ADAMTS‑7 and cartilage oligomeric matrix protein (COMP) in mice with lipopolysaccharide (LPS)‑induced abortion (10 mice/group), and in SA humans and the corresponding control group (21 participants in the SA group and 15 participants in the control group). The results revealed that ADAMTS‑7 expression was upregulated and that COMP expression was downregulated in the mouse decidual tissue of the LPS‑induced abortion group, when compared with that of the normal control group. The results were further confirmed by western blot analysis and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) analysis, which revealed increased ADAMTS‑7 and decreased COMP expression at the protein and mRNA levels in mice treated with LPS. Additionally, the expression of ADAMTS‑7 was negatively correlated with the expression of COMP in mice, with a correlation coefficient of ‑0.936 (P<0.001). In addition, the expression of ADAMTS‑7 and COMP exhibited was similar in the decidual tissue of SA patients when compared with the levels observed in the tissues of the normal control participants, as demonstrated by increased ADAMTS‑7 expression and decreased COMP expression. Western blotting and RT‑qPCR analysis revealed that ADAMTS‑7 was increased and COMP was decreased in the decidual tissue of SA subjects. The correlation analysis of ADAMTS‑7 and COMP in human decidual tissue also revealed a similar result, with a correlation coefficient of ‑0.836 (P<0.001). The results of the present study demonstrated that ADAMTS‑7 was upregulated and COMP was downregulated in the decidual tissues of humans and mice with SA, and a negative correlation was identified between the expression levels of ADAMTS‑7 and COMP, thereby providing novel evidence for a better understanding of the pathogenesis of SA, which may lead to improvements in the clinical pregnancy outcomes of these individuals.
解整合素金属蛋白酶 7（ADAMTS-7）已被证实在炎症相关疾病中发挥重要作用。然而，ADAMTS-7 在自然流产（SA）中的作用尚不清楚。在本研究中，使用人蜕膜组织和小鼠蜕膜组织检测脂多糖（LPS）诱导流产（每组 10 只小鼠）和 SA 人类及相应对照组（SA 组 21 例，对照组 15 例）中小鼠蜕膜组织中 ADAMTS-7 和软骨寡聚基质蛋白（COMP）的表达。结果显示，与正常对照组相比，LPS 诱导流产组小鼠蜕膜组织中 ADAMTS-7 表达上调，COMP 表达下调。Western blot 分析和逆转录-定量聚合酶链反应（RT-qPCR）分析进一步证实了这一点，结果显示 LPS 处理的小鼠中 ADAMTS-7 蛋白和 mRNA 水平升高，COMP 表达降低。此外，ADAMTS-7 与 COMP 在 LPS 处理的小鼠中的表达呈负相关，相关系数为-0.936（P<0.001）。此外，与正常对照组相比，SA 患者蜕膜组织中 ADAMTS-7 和 COMP 的表达相似，表现为 ADAMTS-7 表达增加，COMP 表达降低。Western blot 和 RT-qPCR 分析显示，SA 患者蜕膜组织中 ADAMTS-7 增加，COMP 减少。人蜕膜组织中 ADAMTS-7 和 COMP 的相关性分析也得到了类似的结果，相关系数为-0.836（P<0.001）。本研究结果表明，SA 患者和小鼠蜕膜组织中 ADAMTS-7 表达上调，COMP 表达下调，ADAMTS-7 和 COMP 的表达水平呈负相关，为更好地理解 SA 的发病机制提供了新的证据，可能改善这些患者的临床妊娠结局。
