miR-122-5p 通过靶向 SATB1 抑制鼻咽癌细胞的增殖、迁移和侵袭。
MiR-122-5p suppresses cell proliferation, migration and invasion by targeting SATB1 in nasopharyngeal carcinoma.
机构信息
Department of Otorhinolaryngology Head and Neck Surgery, Second Affiliated Hospital of Nanchang University, Nanchang, P.R. China.
出版信息
Eur Rev Med Pharmacol Sci. 2019 Jan;23(2):622-629. doi: 10.26355/eurrev_201901_16876.
OBJECTIVE
Mounting evidence has suggested that microRNAs (miRNAs) play crucial roles in the progression of nasopharyngeal carcinoma (NPC). However, the molecular mechanism remains not fully understood. We aimed to examine the expression and biological function of miR-122-5p in NPC.
MATERIALS AND METHODS
Quantitative Real Time-Polymerase Chain Reaction was conducted to examine the expression of miR-122-5p. Cell Counting Kit-8 assay, colony formation assay, wound healing assay and transwell assay were performed to measure cell proliferation, colony formation, migration and invasion. Luciferase reporter assay and Western blot assay were used to confirm the target gene of miR-122-5p.
RESULTS
The results showed that miR-122-5p was significantly downregulated in NPC cell lines. Additionally, it was demonstrated that special AT-rich sequence-binding protein 1 (SATB1) was targeted by miR-122-5p. Furthermore, our results revealed that miR-122-5p inhibits cell proliferation, colony formation, cell migration and cell invasion by targeting SATB1.
CONCLUSIONS
Our data suggested that miR-122-5p functions as a tumor suppressor and may be a therapeutic target for NPC.
目的
越来越多的证据表明 microRNAs（miRNAs）在鼻咽癌（NPC）的进展中发挥着关键作用。然而，其分子机制仍不完全清楚。本研究旨在研究 miR-122-5p 在 NPC 中的表达和生物学功能。
材料与方法
采用实时定量聚合酶链反应检测 miR-122-5p 的表达。通过细胞计数试剂盒-8 检测、集落形成实验、划痕愈合实验和 Transwell 实验来测量细胞增殖、集落形成、迁移和侵袭。通过荧光素酶报告基因实验和 Western blot 实验来验证 miR-122-5p 的靶基因。
结果
结果表明，miR-122-5p 在 NPC 细胞系中表达明显下调。此外，证实特殊 AT 富含序列结合蛋白 1（SATB1）是 miR-122-5p 的靶基因。进一步的研究结果表明，miR-122-5p 通过靶向 SATB1 抑制细胞增殖、集落形成、细胞迁移和细胞侵袭。
结论
我们的数据表明，miR-122-5p 作为一种肿瘤抑制因子发挥作用，可能成为 NPC 的治疗靶点。
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