一种用于检测人血清和脑脊液中γ-γ烯醇化酶活性的免疫生物发光测定法。

An immunobioluminescence assay for gamma-gamma enolase activity in human serum and cerebrospinal fluid.

作者信息

Wevers R A, Theunisse A W, Rijksen G

机构信息

Institute of Neurology, University Hospital Nijmegen, The Netherlands.

出版信息

Clin Chim Acta. 1988 Dec 15;178(2):141-50. doi: 10.1016/0009-8981(88)90220-3.

DOI:10.1016/0009-8981(88)90220-3

PMID:3072116

Abstract

A solid phase immunobioluminescence assay for the measurement of gamma-gamma enolase activity in human serum and cerebrospinal fluid is described. The assay combines the specificity of the antiserum with the specificity of measuring enolase activity, thus making the assay highly specific for gamma-gamma enolase. The sensitivity of the method allows determinations in unconcentrated cerebrospinal fluid samples. The new immunobioluminescence assay has been compared with a radioimmunoassay method. The correlation is good both for serum and CSF samples. However, different correlation lines are found for serum and CSF.

摘要

描述了一种用于测量人血清和脑脊液中γ-γ烯醇化酶活性的固相免疫生物发光测定法。该测定法将抗血清的特异性与测量烯醇化酶活性的特异性相结合，从而使该测定法对γ-γ烯醇化酶具有高度特异性。该方法的灵敏度允许对未浓缩的脑脊液样本进行测定。已将新的免疫生物发光测定法与放射免疫测定法进行了比较。血清和脑脊液样本的相关性都很好。然而，血清和脑脊液的相关线不同。