Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Hunan University, Changsha, 410082, People's Republic of China.
College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, 450001, People's Republic of China.
Mikrochim Acta. 2019 Feb 5;186(3):160. doi: 10.1007/s00604-019-3262-7.
Strongly fluorescent polymers (FPs) were prepared from citric acid and ethylenediamine via a hydrothermal approach. The FPs display low toxicity, water solubility, a quantum yield of 91%, good photostability and stability in the physiological pH range. Ferric ions are found to quench the fluorescence which is best measured at excitation/emission wavelengths of 350/440 nm. Because ferric ions (Fe) can quench the fluorescence of FPs, a fluorometric method was developed for fast detection of Fe and within 1 min. FPs can also be used indirectly for the detection of hydrogen peroxide because of its fast Fenton reaction with Fe to generate of Fe. The detection limits are 8 μM for Fe(III) and 0.6 μM for HO. On the basis of the glucose oxidase catalyzed of glucose and the Fenton reaction, the FPs can also be used to quantify glucose with a linear response in the 0.5-10 μM concentration range. Graphical abstract A new type of polymer with high fluorescence quantum yield was prepared. It is shown to enable the fast detection of ferric ions, hydrogen peroxide and glucose based on quenching and on the glucose oxidase and Fenton reactions.
通过水热法，从柠檬酸和乙二胺制备出强荧光聚合物（FPs）。FPs 具有低毒性、水溶性、91%的量子产率、良好的光稳定性和在生理 pH 范围内的稳定性。铁离子被发现会猝灭荧光，最佳的荧光测量激发/发射波长为 350/440nm。由于铁离子（Fe）可以猝灭 FPs 的荧光，因此开发了一种荧光法用于快速检测 Fe，检测时间在 1 分钟内。由于其与 Fe 的快速 Fenton 反应生成 Fe，FPs 也可间接用于检测过氧化氢。Fe(III)的检测限为 8μM，HO 的检测限为 0.6μM。基于葡萄糖氧化酶催化的葡萄糖和 Fenton 反应，FPs 还可以用于定量检测葡萄糖，在 0.5-10μM 浓度范围内具有线性响应。
