Department of Environmental Engineering and Green Technology, Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia, Jalan Sultan Yahya Petra, 54100 Kuala Lumpur, Malaysia.
Analytical and Scientific Instrument Division, Shimadzu Malaysia Sdn. Bhd. Nouvelle Industrial Park 2, Taman Sains Selangor 1, Kota Damansara, 47810 Petaling Jaya, Selangor, Malaysia.
Bioresour Technol. 2019 May;279:174-180. doi: 10.1016/j.biortech.2019.01.122. Epub 2019 Jan 26.
Previous studies on screening of lignin-degrading bacteria mainly focused on the ligninolytic ability of the isolated bacteria for the utilization of lignin monomers. In this study, we focused on the depolymerization of alkali lignin to prove the ability of the isolated thermophilic bacterial strains to utilize and depolymerize more than a monomer of alkali lignin within 7 days of incubation. Indigenous thermophilic bacterial isolates from the palm oil plantation were used to evaluate the depolymerization and utilization of alkali lignin. The confirmation of the bacterium-mediated depolymerization of oil palm empty fruit bunch was achieved through the removal of silica bodies, as observed with scanning electron microscopy. Stenotrophomonas sp. S2 and Bacillus subtilis S11Y were able to reduce approximately 50% and 20% of alkali lignin at 7 days of incubation without the requirement for additional carbon sources.
先前关于木质素降解菌筛选的研究主要集中在分离菌对木质素单体的利用的木质素降解能力上。在本研究中，我们关注的是碱木质素的解聚，以证明分离出的嗜热细菌菌株在 7 天的孵育时间内利用和解聚不止一种碱木质素单体的能力。从棕榈油种植园中分离出的土著嗜热细菌被用来评估碱木质素的解聚和利用。通过扫描电子显微镜观察到硅体的去除，证实了细菌介导的油棕空果串的解聚。在 7 天的孵育时间内，不动杆菌 S2 和枯草芽孢杆菌 S11Y 可以减少约 50%和 20%的碱木质素，而不需要额外的碳源。
