Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Malaysia.
Laboratory of Biopolymer and Derivatives, Institute of Tropical Forestry and Forest Products, Universiti Putra Malaysia, 43400 UPM Serdang, Malaysia.
Molecules. 2018 Apr 2;23(4):811. doi: 10.3390/molecules23040811.
The combination of superheated steam (SHS) with ligninolytic enzyme laccase pretreatment together with size reduction was conducted in order to enhance the enzymatic hydrolysis of oil palm biomass into glucose. The oil palm empty fruit bunch (OPEFB) and oil palm mesocarp fiber (OPMF) were pretreated with SHS and ground using a hammer mill to sizes of 2, 1, 0.5 and 0.25 mm before pretreatment using laccase to remove lignin. This study showed that reduction of size from raw to 0.25 mm plays important role in lignin degradation by laccase that removed 38.7% and 39.6% of the lignin from OPEFB and OPMF, respectively. The subsequent saccharification process of these pretreated OPEFB and OPMF generates glucose yields of 71.5% and 63.0%, which represent a 4.6 and 4.8-fold increase, respectively, as compared to untreated samples. This study showed that the combination of SHS with laccase pretreatment together with size reduction could enhance the glucose yield.
为了提高棕榈油生物质转化为葡萄糖的酶水解效率，采用过热蒸汽（SHS）与木质素降解酶漆酶预处理相结合并辅以减小粒径的方法。将油棕空果串（OPEFB）和油棕中果皮纤维（OPMF）先用 SHS 预处理，然后用锤磨机粉碎至 2、1、0.5 和 0.25mm 粒径，再用漆酶去除木质素。研究表明，从原料减小至 0.25mm 对漆酶去除木质素有重要作用，分别去除了 38.7%和 39.6%的 OPEFB 和 OPMF 木质素。对这些预处理过的 OPEFB 和 OPMF 进行后续糖化处理，可分别产生 71.5%和 63.0%的葡萄糖产量，与未处理样品相比，分别提高了 4.6 倍和 4.8 倍。本研究表明，过热蒸汽与漆酶预处理相结合并辅以减小粒径可提高葡萄糖产量。
