Matos Luis A, Hilf Mark E, Cayetano Xiomara A, Feliz Andrea O, Harper Scott J, Folimonova Svetlana Y
Citrus Research and Education Center, University of Florida, Lake Alfred, FL 33850, and Instituto Dominicano de Investigaciones Agropecuarias y Forestales (IDIAF), Santo Domingo, Dominican Republic.
United States Department of Agriculture-Agricultural Research Service USHRL, Fort Pierce, FL 34945.
Plant Dis. 2013 Mar;97(3):339-345. doi: 10.1094/PDIS-05-12-0421-RE.
Citrus tristeza virus (CTV) is the most destructive viral pathogen of citrus and has been an important concern for the citrus industry in the Dominican Republic. Earlier studies documented widespread distribution of mild isolates of the T30 genotype, which caused no disease in the infected trees, and a low incidence of isolates of the VT and T3 genotypes, which were associated with economically damaging decline and stem-pitting symptoms in sweet orange and Persian lime, the two major citrus varieties grown in the Dominican Republic. In light of the dramatic increase in the number of severely diseased citrus trees throughout the country over the last decade, suggesting that field populations of CTV have changed, we examined the CTV pathosystem in the Dominican Republic to assess the dynamics of virus populations. In this work, we characterized the molecular composition of 163 CTV isolates from different citrus-growing regions. Our data demonstrate a dramatic change in CTV populations, with the VT genotype now widely disseminated throughout the different regions and with the presence of two new virus genotypes, T36 and RB. Multiple infections of trees resulted in development of complex virus populations composed of different genotypes.
柑橘衰退病毒（CTV）是柑橘最具毁灭性的病毒病原体，一直是多米尼加共和国柑橘产业的重要关切问题。早期研究记录了T30基因型温和分离株的广泛分布，这些分离株在受感染的树木中不会引发疾病，以及VT和T3基因型分离株的低发生率，这些分离株与多米尼加共和国种植的两个主要柑橘品种甜橙和波斯青柠中具有经济破坏性的衰退和茎陷点症状有关。鉴于过去十年间全国严重患病柑橘树数量急剧增加，这表明CTV的田间种群发生了变化，我们对多米尼加共和国的CTV病理系统进行了研究，以评估病毒种群的动态。在这项工作中，我们对来自不同柑橘种植区的163个CTV分离株的分子组成进行了表征。我们的数据表明CTV种群发生了巨大变化，VT基因型现已广泛传播到不同地区，并且出现了两种新的病毒基因型T36和RB。树木的多重感染导致了由不同基因型组成的复杂病毒种群的形成。
