Seifers D L, Martin T J, Haber S
Professor.
Professor, Kansas State University Agricultural Research Center-Hays, Hays 67601.
Plant Dis. 2013 Jul;97(7):983-987. doi: 10.1094/PDIS-10-12-0971-RE.
Temperature-sensitive resistance (TSR) that can protect against losses to Wheat streak mosaic virus (WSMV) has been described in elite wheat germplasm. A TSR identified in the advanced breeding line CO960333 and its derivative KS06HW79 was examined in growth-chamber tests conducted under constant temperature regimes of 18, 21, and 24°C against an array of WSMV isolates. At 18°C, all tested isolates systemically infected the pedigree parents, while the progeny line CO960333 remained free of symptoms; at 24°C, all lines were susceptible. At the intermediate temperature of 21°C, the TSR of KS06HW79 was effective in contrast to the TSRs of KS03HW12 and 'RonL'. In field trials conducted in 2011 and 2012, the TSR expressed in KS06HW79 conferred complete protection against yield losses from inoculation with the Sidney 81 isolate of WSMV, while the TSR of RonL conferred similar protection in 2012 but allowed small losses in 2011. The resistance expressed by KS06HW79 is likely not due to the Wsm1 gene because it did not contain the tightly linked J15 sequence-characterized amplified region (SCAR) DNA marker. These findings suggest that KS06HW79 could be an additional TSR source of value to wheat-breeding programs seeking to control losses from WSMV.
在优良小麦种质中已发现对小麦线条花叶病毒（WSMV）具有保护作用的温度敏感抗性（TSR）。在高级育种系CO960333及其衍生品种KS06HW79中鉴定出的一种TSR，在18、21和24°C恒温条件下进行的生长室试验中，针对一系列WSMV分离株进行了检测。在18°C时，所有测试分离株均系统感染了系谱亲本，而后代品系CO960333仍无症状；在24°C时，所有品系均易感。在21°C的中间温度下，KS06HW79的TSR有效，而KS03HW12和“RonL”的TSR则无效。在2011年和2012年进行的田间试验中，KS06HW79中表达的TSR对接种WSMV的Sidney 81分离株造成的产量损失提供了完全保护，而RonL的TSR在2012年提供了类似的保护，但在2011年造成了小的产量损失。KS06HW79所表达的抗性可能不是由于Wsm1基因，因为它不包含紧密连锁的J15序列特征性扩增区域（SCAR）DNA标记。这些发现表明，KS06HW79可能是寻求控制WSMV造成损失的小麦育种计划的另一个有价值的TSR来源。
