Sinnis P, Wellems T E
Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
Genomics. 1988 Nov;3(4):287-95. doi: 10.1016/0888-7543(88)90117-6.
Homologous chromosomes from the human malaria parasite Plasmodium falciparum exhibit striking size polymorphism from isolate to isolate. To examine the structural basis for these variations, we have determined full-length restriction maps of chromosome 4 from three P. falciparum clones. Two clones, HB3 and 3D7, are derived from geographically distant strains, while the third, XP5, is the product of an HB3/3D7 cross. The restriction maps show that, while the overall structure and organization of chromosome 4 from each clone are similar, large-scale variations occur within a few hundred kilobase pairs of the chromosome ends. An apparent crossover between the 3D7 and the HB3 parent chromosomes accounts for a chromosome of intermediate size in clone XP5. Similar restriction studies extended to other parasite chromosomes will ultimately yield a long-range physical map of the P. falciparum genome.
来自人类疟原虫恶性疟原虫的同源染色体在不同分离株之间表现出显著的大小多态性。为了研究这些变异的结构基础，我们确定了来自三个恶性疟原虫克隆的4号染色体的全长限制性图谱。两个克隆HB3和3D7来自地理上遥远的菌株，而第三个克隆XP5是HB3/3D7杂交的产物。限制性图谱表明，虽然每个克隆的4号染色体的总体结构和组织相似，但在染色体末端的几百千碱基对内发生了大规模变异。3D7和HB3亲本染色体之间明显的交叉导致了克隆XP5中出现中等大小的染色体。扩展到其他寄生虫染色体的类似限制性研究最终将产生恶性疟原虫基因组的长程物理图谱。
