体外暴露于长波紫外线照射后大鼠心脏和肾脏细胞上主要组织相容性复合体II类抗原表达降低。
Reduced MHC class II antigen expression on rat heart and kidney cells after in vitro exposure to longwave ultraviolet irradiation.
作者信息
Eichler C, Oesterwitz H, Kaden J, Schneider W, Schade R, Scholz D
机构信息
Department of Urology, Humboldt-University of Berlin, G.D.R.
出版信息
Immunol Lett. 1988 Dec;19(4):273-8. doi: 10.1016/0165-2478(88)90154-x.
The pretreatment of both kidney and heart donor with the photosensitizer 8-methoxy-psoralen followed by in vitro longwave ultraviolet graft irradiation (PUVA therapy) significantly prolonged graft survival times in rats even across a strong major histocompatibility complex (MHC) barrier. Immunohistological studies using monoclonal antibodies (Mab) to rat MHC class I and II antigens showed a significant reduction of class II antigen expression after PUVA therapy in both Sprague-Dawley rat kidneys and hearts. Using MHC class I Mab there were no differences in distribution pattern in untreated as well as in PUVA treated organs. Thus, our results represent the first in vivo evidence that photochemotherapy-induced graft survival time prolongation is closely connected with its ability to reduce MHC class II antigen expression.
用光敏剂8-甲氧基补骨脂素预处理肾脏和心脏供体，随后进行体外长波紫外线移植物照射（补骨脂素加紫外线A疗法），即使跨越强大的主要组织相容性复合体（MHC）屏障，也能显著延长大鼠移植物的存活时间。使用针对大鼠MHC I类和II类抗原的单克隆抗体（Mab）进行的免疫组织学研究表明，在补骨脂素加紫外线A疗法后，斯普拉格-道利大鼠的肾脏和心脏中II类抗原表达显著降低。使用MHC I类Mab，未处理器官和补骨脂素加紫外线A处理器官的分布模式没有差异。因此，我们的结果首次提供了体内证据，表明光化学疗法诱导的移植物存活时间延长与其降低MHC II类抗原表达的能力密切相关。
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