Fickler Anna, Staats Stefanie, Michl Stéphanie Céline, Hasler Mario, Rimbach Gerald, Schulz Carsten
GMA - Gesellschaft für Marine Aquakultur mbH, Hafentörn 3, 25761, Büsum, Germany.
Institute of Animal Breeding and Husbandry, Kiel University, Hermann-Rodewald-Str. 6, 24118, Kiel, Germany.
Lipids. 2018 Nov;53(11-12):1069-1083. doi: 10.1002/lipd.12117. Epub 2019 Feb 5.
Equol and Ahiflower oil have been shown to increase either eicosapentaenoic acid (EPA, 20:5n-3) or docosahexaenoic acid (DHA, 22:6n-3) levels in tissues of rainbow trout when applied individually. Thus, we investigated whether the combination of an Ahiflower oil-based diet and equol might increase both, EPA and DHA levels, in rainbow trout. Rainbow trout (87.1 ± 0.3 g) were fed with five diets for 8 weeks. A diet based on a blend of fish and vegetable oils (FV) served as a reference diet. The four experimental diets contained a blend of Ahiflower oil and vegetable oils (AV). The AV-diets were supplemented with equol by 0.0%, 0.1%, 0.2%, and 0.3% DM of the diet (AV-C, AV-EQ1, AV-EQ2, and AV-EQ3). The dietary treatments did not affect growth performance of fish and chemical nutrient composition of the whole body samples. Fish fed with the equol diets showed dose-dependently increased liver weights and 18:0 liver levels. The content of EPA showed no consistent pattern between tissues but all AV-groups were characterized by higher liver EPA values than FV. DHA values of AV-EQ2 and AV-EQ3 were similar to FV in fillet, tended to be the highest in the whole body and were significantly higher in liver compared to FV. In contrast, mRNA steady state levels of fatty acyl desaturase 2a (delta-6) [fads2a(d6)] were not affected by the dietary treatments. In conclusion, the combination of dietary Ahiflower oil and equol (0.2% and 0.3%) seems to affect the fatty acid metabolism of rainbow trout positively to increase DHA tissue levels.
已证明，单独使用雌马酚和高活性红花油时，均可提高虹鳟鱼组织中的二十碳五烯酸（EPA，20:5n-3）或二十二碳六烯酸（DHA，22:6n-3）水平。因此，我们研究了以高活性红花油为基础的日粮与雌马酚联合使用是否能提高虹鳟鱼体内的EPA和DHA水平。将虹鳟鱼（87.1±0.3克）用五种日粮喂养8周。以鱼和植物油混合物（FV）为基础的日粮作为对照日粮。四种实验日粮含有高活性红花油和植物油的混合物（AV）。在AV日粮中分别添加0.0%、0.1%、0.2%和0.3%日粮干物质的雌马酚（AV-C、AV-EQ1、AV-EQ2和AV-EQ3）。日粮处理对鱼的生长性能和全身样本的化学营养成分没有影响。饲喂含雌马酚日粮的鱼肝脏重量和肝脏中18:0水平呈剂量依赖性增加。EPA含量在各组织间无一致规律，但所有AV组肝脏中的EPA值均高于FV组。AV-EQ2和AV-EQ3组的鱼片DHA值与FV组相似，在全身中往往最高，且肝脏中的DHA值显著高于FV组。相比之下，日粮处理对脂肪酸去饱和酶2a（delta-6）[fads2a(d6)]的mRNA稳态水平没有影响。总之，日粮中高活性红花油和雌马酚（0.2%和0.3%）联合使用似乎能积极影响虹鳟鱼的脂肪酸代谢，从而提高组织中的DHA水平。
