Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China; Key Laboratory of Functional Dairy, Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China; Department of Animal Sciences, Washington State University, Pullman, WA 99164, USA.
Food Chem. 2019 Mar 1;275:59-68. doi: 10.1016/j.foodchem.2018.09.044. Epub 2018 Sep 8.
The aim of the present study was to compare the different bioactivities of milk fat globule membrane (MFGM) proteins between yak and cow, and identified the differential MFGM proteins. In this study, 156 differential MFGM proteins between yak and cow were identified with various health-related bioactivities including anti-adhesion of bacteria and viruses, glucose and lipid metabolism, immunoregulation, antioxidant activity, anti-cancer and neural regulation by GOBP and KEGG pathway analysis. The efficacy of differential MFGM proteins from cow and yak milk on glucose and lipid metabolism was verified by in vitro HepG2 cells. MFGM proteins of yak exhibited significantly higher lipid accumulation-lowering efficacy, and MFGM proteins of cow had significantly higher efficacy of increasing glucose uptake. The differential types and amount MFGM proteins of yak and cow milk that might relate to the different effect on glucose and lipid metabolism were also identified. These findings demonstrate that MFGM proteins contain potential bioactive ingredients for improving metabolic health.
本研究旨在比较牦牛和奶牛乳脂肪球膜（MFGM）蛋白的不同生物活性，并鉴定差异 MFGM 蛋白。本研究通过 GOBP 和 KEGG 通路分析，共鉴定出 156 种具有不同健康相关生物活性的牦牛和奶牛差异 MFGM 蛋白，包括抗细菌和病毒黏附、葡萄糖和脂质代谢、免疫调节、抗氧化活性、抗癌和神经调节。通过体外 HepG2 细胞验证了来自牦牛奶和奶牛乳的差异 MFGM 蛋白对葡萄糖和脂质代谢的功效。牦牛 MFGM 蛋白表现出显著更高的降低脂质积累的功效，而奶牛 MFGM 蛋白具有显著更高的增加葡萄糖摄取的功效。还鉴定了与葡萄糖和脂质代谢不同作用相关的牦牛和奶牛乳中差异类型和数量的 MFGM 蛋白。这些发现表明 MFGM 蛋白含有改善代谢健康的潜在生物活性成分。
