Güler Sertaç Ata, Kırnaz Sertaç, Şimşek Turgay, Demir Can İlker, Güneş Abdullah, İşken Tonguç, Cantürk Nuh Zafer, Utkan Nihat Zafer
General Surgery Department, Kocaeli University School of Medicine, Kocaeli, Turkey.
Plastic and Reconstructive Surgery Department, Kocaeli University School of Medicine, Kocaeli, Turkey.
Acta Chir Belg. 2020 Jun;120(3):167-172. doi: 10.1080/00015458.2019.1570742. Epub 2019 Feb 6.
Sentinel lymph node biopsy is important for metastasis surveillance in the management of a number of human cancers. Identification of sentinel lymph nodes may be facilitated by the use of several methods including methylene blue injection. However tissue necrosis is a known effect of methylene blue application. This study aimed to investigate the effects of methylene blue injection on skin flaps using a rodent model. Modified McFarlane flaps were prepared using Wistar Albino rats. Local injection of methylene blue was given to one group while saline was injected into the control group. A third group received systemic methylene blue via intraperitoneal injection. Observational and histological comparison was made between the groups to investigate the necrotic effects of methylene blue on skip flaps. The control group's surviving flap areas were significantly larger than local methylene blue group's surviving flap area. However, there was no significant difference in skin flap survival area between the control group and the systemic methylene blue group. Furthermore, there is no significant difference between local and systemic methylene blue group's surviving flap areas. Local methylene blue injection increases skin necrosis and decreases rates of surviving flap areas in an experimental rat models.
前哨淋巴结活检对于多种人类癌症治疗中的转移监测至关重要。使用包括注射亚甲蓝在内的多种方法有助于识别前哨淋巴结。然而，组织坏死是应用亚甲蓝已知的一种效应。本研究旨在使用啮齿动物模型研究亚甲蓝注射对皮瓣的影响。采用Wistar白化大鼠制备改良的麦克法兰皮瓣。一组进行局部亚甲蓝注射，而对照组注射生理盐水。第三组通过腹腔注射接受全身亚甲蓝。对各组进行观察和组织学比较，以研究亚甲蓝对随意皮瓣的坏死效应。对照组存活皮瓣面积显著大于局部亚甲蓝组的存活皮瓣面积。然而，对照组与全身亚甲蓝组之间皮瓣存活面积无显著差异。此外，局部和全身亚甲蓝组的存活皮瓣面积之间也无显著差异。在实验大鼠模型中，局部注射亚甲蓝会增加皮肤坏死并降低皮瓣存活面积率。
