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人腺苷脱氨酶在小鼠造血细胞中的表达。

Expression of human adenosine deaminase in murine hematopoietic cells.

作者信息

Belmont J W, MacGregor G R, Wager-Smith K, Fletcher F A, Moore K A, Hawkins D, Villalon D, Chang S M, Caskey C T

机构信息

Institute for Molecular Genetics, Baylor College of Medicine, Houston, Texas 77030.

出版信息

Mol Cell Biol. 1988 Dec;8(12):5116-25. doi: 10.1128/mcb.8.12.5116-5125.1988.

DOI:10.1128/mcb.8.12.5116-5125.1988
PMID:3072474
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC365613/
Abstract

Multiple replication-defective retrovirus vectors were tested for their ability to transfer and express human adenosine deaminase in vitro and in vivo in a mouse bone marrow transplantation model. High-titer virus production was obtained from vectors by using both a retrovirus long terminal repeat promoter and internal transcriptional units with human c-fos and herpes virus thymidine kinase promoters. After infection of primary murine bone marrow with one of these vectors, human adenosine deaminase was detected in 60 to 85% of spleen colony-forming units and in the blood of 14 of 14 syngeneic marrow transplant recipients. This system offers the opportunity to assess methods for increasing efficiency of gene transfer, for regulation of expression of foreign genes in hematopoietic progenitors, and for long-term measurement of the stability of expression in these cells.

摘要

在小鼠骨髓移植模型中，对多种复制缺陷型逆转录病毒载体在体外和体内转移并表达人腺苷脱氨酶的能力进行了测试。通过使用逆转录病毒长末端重复启动子以及带有人类c-fos和疱疹病毒胸苷激酶启动子的内部转录单元，从载体中获得了高滴度病毒。用这些载体之一感染原代小鼠骨髓后，在60%至85%的脾集落形成单位以及14只同基因骨髓移植受体中的14只的血液中检测到了人腺苷脱氨酶。该系统为评估提高基因转移效率的方法、调节造血祖细胞中外源基因表达的方法以及长期测量这些细胞中表达稳定性的方法提供了机会。

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引用本文的文献

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本文引用的文献

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Bone marrow transplantation only partially restores purine metabolites to normal in adenosine deaminase-deficient patients.对于腺苷脱氨酶缺乏症患者，骨髓移植只能使嘌呤代谢产物部分恢复正常。
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Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus.逆转录病毒包装突变体的构建及其用于产生无辅助病毒的缺陷型逆转录病毒。
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Selective overproduction of adenosine deaminase in cultured mouse cells.
Amplified and tissue-directed expression of retroviral vectors using ping-pong techniques.
利用乒乓技术实现逆转录病毒载体的扩增及组织定向表达。
J Mol Med (Berl). 1995 Mar;73(3):113-20. doi: 10.1007/BF00198238.
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Combination of interleukins 3 and 6 preserves stem cell function in culture and enhances retrovirus-mediated gene transfer into hematopoietic stem cells.白细胞介素3和白细胞介素6联合使用可在培养中维持干细胞功能，并增强逆转录病毒介导的基因导入造血干细胞的能力。
Proc Natl Acad Sci U S A. 1989 Nov;86(22):8897-901. doi: 10.1073/pnas.86.22.8897.
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Long-term expression of human adenosine deaminase in mice transplanted with retrovirus-infected hematopoietic stem cells.人腺苷脱氨酶在移植了逆转录病毒感染的造血干细胞的小鼠中的长期表达。
Proc Natl Acad Sci U S A. 1989 Nov;86(22):8892-6. doi: 10.1073/pnas.86.22.8892.
6
Retroviral vector-mediated high-efficiency expression of adenosine deaminase (ADA) in hematopoietic long-term cultures of ADA-deficient marrow cells.逆转录病毒载体介导的腺苷脱氨酶（ADA）在ADA缺陷骨髓细胞造血长期培养物中的高效表达。
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Expression of human alpha-globin and mouse/human hybrid beta-globin genes in murine hemopoietic stem cells transduced by recombinant retroviruses.重组逆转录病毒转导的小鼠造血干细胞中人α-珠蛋白和小鼠/人杂交β-珠蛋白基因的表达
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培养的小鼠细胞中腺苷脱氨酶的选择性过量产生。
J Biol Chem. 1983 Jul 10;258(13):8338-45.
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A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.一种将DNA限制性内切酶片段放射性标记至高比活度的技术。
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Structure of adenosine deaminase mRNAs from normal and adenosine deaminase-deficient human cell lines.来自正常和腺苷脱氨酶缺陷型人类细胞系的腺苷脱氨酶mRNA的结构
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Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.变性RNA与转移至硝酸纤维素膜上的小DNA片段的杂交。
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