Hsu Myriam Y, Porporato Paolo E, Wyart Elisabeth
Department of Molecular Biotechnology and Health Science, Molecular Biotechnology Center, University of Torino, Torino, Italy.
Methods Mol Biol. 2019;1928:337-352. doi: 10.1007/978-1-4939-9027-6_17.
Cancer cachexia is a metabolic disease characterized by a negative energy balance associated with systemic weight loss and poor quality of life.In particular, skeletal muscle, which represents almost 50% of the total body mass, is strongly affected, and metabolic alterations therein (e.g., insulin resistance and mitochondrial dysfunction) can eventually support tumor growth by facilitating nutrient scavenging by the growing mass. Interestingly, metabolic interventions on wasting muscle have been proven to be protective, advocating for the importance of metabolic regulation in the wasting muscle.Here, we will briefly define the current knowledge of metabolic regulation in cachexia and provide a protocol to grow and differentiate in vitro myotubes for the assessment of mitochondrial metabolism during cachexia.
癌症恶病质是一种代谢性疾病，其特征为负能量平衡，伴有全身体重减轻和生活质量下降。特别是，占全身质量近50%的骨骼肌受到严重影响，其中的代谢改变（如胰岛素抵抗和线粒体功能障碍）最终可通过促进生长中的肿瘤组织清除营养物质来支持肿瘤生长。有趣的是，对消瘦肌肉进行代谢干预已被证明具有保护作用，这表明代谢调节在消瘦肌肉中具有重要意义。在此，我们将简要阐述目前关于恶病质中代谢调节的认识，并提供一种体外培养和分化肌管的方案，用于评估恶病质期间的线粒体代谢。
